摘要
目的 研究乳腺癌中端粒酶活性的表达 ,探讨端粒酶活化与乳腺癌发生发展的关系及其作为乳腺癌肿瘤标志物的可能性。方法 应用TRAP 银染法及TRAP ELISA方法对 2 7例乳腺癌组织及 18例相应癌旁组织中的端粒酶活性进行了定性及定量测定。结果 在 2 7例乳腺癌组织中 ,发现有 2 3例端粒酶表达阳性 ,阳性率为 85 .2 % ;而 18例相应癌旁组织中只有 1例表达阳性 ;乳腺癌组织中的端粒酶活性 (A值 )为 0 .5 85± 0 .2 86;而相应癌旁组织则为 0 .0 95±0 .0 62 ,两者比较有显著性差异 (P <0 .0 1)。乳腺癌肿块直径≥ 2cm及淋巴结转移阳性者端粒酶活性水平显著高于 <2cm及淋巴结转移阴性者 (P <0 .0 5 ) ;而端粒酶活性与肿瘤的病理类型无明显相关性 (P >0 .0 5 )。结论 端粒酶活化在乳腺癌的发生发展中起重要作用 ,有可能成为乳腺癌早期诊断的理想肿瘤标志物。
Objective To investigate the telomerase activity in human breast cancers and their adjacent tissues and to explore the possible relationship between telomerase expression and progression of breast cancer and the possibility of telomerase as a specific tumor maker.Methods Qualitative and quantitative analysis of telomerase activity were detected by sliver staining telmeric repeat amplification protocol(TRAP) and TRAP ELISA assays in 27 cases of primary breast cancer and 18 tumor adjancent tissues.Results Telomerase activity were detected in 24 of 27 human breast cancer samples with an 85.2% positive rate,but in only 1 of 18 tumor adjacent tissues (P<0.01).The levels of telomerase activity in breast cancer and tumor adjacent tissues were 0.585±0.286 A and 0.095±0.062 A respectively,and the difference was significant in statistics (P<0.01).The telomerase activity levels in breast cancers with the diameter of the tumor ≥ 2cm and axillary lymphatic metastasis were higher than those in the group of the tumor diameter <2cm and without lymphatic metastasis (P<0.05).However,no significant correlation was found between telomerase expression and the pathological type of breast carcinomas(P>0.05).Conclusion Telomerase activation might play an important role in the developemnt of human breast carcinomas,and it could be used as a tumor marker in the early diagnosis of breast carcinoma.
出处
《中国肿瘤临床与康复》
2002年第6期21-22,20,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
