摘要
目的:建立和改进香砂养胃丸含量测定方法。方法:以Hypersil ODS C_(18)(5μm,150 mm×4.6mm)色谱柱分离厚朴酚 与和厚朴酚,流动相乙腈-水-冰醋酸(50:8:2),检测波长294nm。结果:线性范围:厚朴酚1.12～5.60μg,r=0.9999(n=5),和厚朴酚0.8288～4.144μg,r=0.9999(n=5)。厚朴酚平均回收率99.1％,RSD=1.64％(n=5),和厚朴酚平均回收率102.4％,RSD=1.67％(n=5)。结论:本法简便,快速,准确,可作为该制剂质量控制的方法。
OBJECTIVE To establish and improve an HPLC method for the determination of magnolol and isomagnolol in Xiang-sha Yangwei pills. METHODS Magnolol and iso-magnolol were separated on Hvpersil ODS C18< column( 5μm, 150mm×4.6mm) and detected at 294 nm, using acetonitrile-water-acelic acid glacial (50:48:2) as mobile phase. RESULTS The standard curves for magnolol and magnolol were linear in t he range of 1. 12-5. 60 μg and 0. 8288 - 4. 144 μg. The average recoveries of magnoiol and iso-magnolol were 99. 1 % (RSD= 1.64% ) and 102.4% (RSD = 1.67 %). CONCLUSIONS The method is simple rapid and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2002年第12期734-736,共3页
Chinese Journal of Hospital Pharmacy
