摘要
目的 探讨荧光定量聚合酶链反应 (FQ PCR)与常规聚合酶链反应检测乙型肝炎病毒 (HBV)敏感性的差异。方法 对 10 5份乙型肝炎患者血清用两种方法检测HBV。结果 常规PCR对 10 6～ 10 9、10 5～ 10 4 和≤ 10 3 copy/ml的血清检测结果有统计学差异。常规PCR检测 10 6～10 9copy/ml的血清假阴性率仅 5 .0 %,10 4 ～ 10 5copy/ml时假阴性率达 36 .8%,10 3 copy/ml主要为阴性结果 ,但有 6例为阳性或弱阳性 ( 2 4.0 %)。结论 常规PCR对≤ 10 5copy/ml的血清有较高的假阴性率 ,比FQ PCR灵敏度低 10～ 10 0倍。弱阳性标本FQ PCR检测也可能出现假阴性结果。
Objective To explore the sensitivity difference between fluorescent quantity PCR (FQ-PCR) and routine PCR for HBV DNA detection.Methods HBV copies in sera from 105 patients with hepatitis B were detected by both FQ-PCR and routine PCR.Results For sera with 10 6～10 9, 10 5～10 4 and <10 3copy HBV/ml,routine PCR and FQ-PCR showed statistical difference.For sera with 10 6～10 9copy HBV/ml,the false negative rate of routine PCR was only 5.0%,while for sera with 10 5～10 4copy HBV/ml,the false negative rate reached 36.8%.In 25 cases with sera HBV equal or less than 10 3 copy/ml,routine PCR showed negative results in most cases except 6 cases (24%) with positive or weak positive results.Conclusion For sera with HBV equal or less than 10 5copy/ml,routine PCR shows higher false negative rate than FQ-PCR,its sensitivity is 10 to 100 times less than FQ-PCR.For sera with weak positive results by routine PCR, FQ-PCR can also show false negative results.
出处
《临床内科杂志》
CAS
北大核心
2003年第1期20-21,共2页
Journal of Clinical Internal Medicine
