摘要
目的 :研究金雀异黄素对氧化型低密度脂蛋白诱导的人血管内皮细胞c mycmRNA表达的影响。方法 :低密度脂蛋白采用密度梯度超速离心法从健康人血浆中提取 ,经铜离子氧化制备成氧化型低密度脂蛋白。培养的人血管内皮细胞给予氧化型低密度脂蛋白 2 0 0mg·L- 1或同时给予金雀异黄素 10 0 μmol·L- 1诱导不同时间 (1,2 ,4h) ,提取细胞总RNA进行Northernblot杂交。结果 :2 0 0mg·L- 1氧化型低密度脂蛋白刺激内皮细胞c mycmR NA在 1h增加为对照水平的 3倍 ,2h增加为对照水平的 3.3倍 ,4h降到对照水平以下 ;给予氧化型低密度脂蛋白诱导的同时给予金雀异黄素 10 0 μmol·L- 1其c mycmRNA表达量在 1h为单独给予氧化型低密度脂蛋白诱导的 80 % ,2h为 6 0 %。结论 :金雀异黄素能有效抑制氧化型低密度脂蛋白诱导的人血管内皮细胞c
AIM: To study the effect of genistein on c myc mRNA expression induced by oxidized low density lipoprotein (ox LDL) in human vascular endothelial cells (ECV304). METHODS: LDL were isolated from healthy human plasma by gradient ultracentrifugation and oxidized by CuSO 4. ECV304 cells were exposed to ox LDL 200 mg·L -1 in the presence or absence of genistein 100 μmol· L -1 for 1, 2, and 4 h in vitro . Northern blot was employed to measure c myc mRNA levels of ECV304. RESULTS: In response to ox LDL 200 mg·L -1 , c myc mRNA expression in ECV304 increased by 3 fold for 1h and 3.3 fold for 2 h and decreased below the control level at 4 h. Expressions of c myc stimulated by ox LDL in the presence of genistein 100 μmol·L -1 for 1 h and 2 h were separately 80 percent and 60 percent of that in the absence of genistein 100 μmol· L -1 . CONCLUSION: Genistein can effectively inhibit c myc mRNA expression in ECV304 induced by ox LDL.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2003年第1期5-8,共4页
Chinese Journal of New Drugs and Clinical Remedies
关键词
血管内皮
金雀异黄素
低密度脂蛋白
genistein
lipoprotein,low density
endothelium, vascular
