摘要
利用随机扩增多态性 DNA(RAPD)技术对 10种早熟禾植物进行了基因组 DNA多态性分析。对 30个OPRON公司十聚体随机引物进行多态性筛选 ,2 8个可产生多态性。 18个引物产生的 2 5 0个 DNA片段 ,用于计算种间的欧氏遗传相似性系数分析 ,在 Statistic统计软件按非加权算术平均数 (U PGMA)进行聚类并构建系统发育树状图。结果表明 ,10种早熟禾植物种间的亲缘关系 ,其变幅主要在 6 .7～ 8.5之间 ;其中 P.botryoides与 P.psilolepis之间的遗传距离最小 ,为 6 .7;其次是 P.sinattenuata与 P.sphondylodes遗传距离较近 ,为 6 .8;而 P.sikkimensis与 P.pratensis,P.sikkimensis与 P.psilolepis之间的距离最远 ,均为 10 .5 ;这与形态学分类结果基本一致 ,说明 RAPD分析方法可从分子生物学角度为早熟禾属植物的系统学分类提供更为有利和可靠的证据。
Ten species of Poa were analyzed using random amplified polymorphic DNA(RAPD) markers. 30 decamer oligonucleotide random primers were used for polymorphic section, among which, 28 primers produced polymorphic RAPD products. A total of 250 bands amplified from 18 primers were selected for RAPD analysis. Jaccard's genetic similarity coefficients were generated and dendrogram was constructed using UPGMA. The result showed that genetic distance between P. botryoides and P. psilolepis was the least, with coefficient 6.7; then the one between P. sinattenuata and P. sphondylodes, with coefficient 6.8; while the genetic distance between P. sikkimensis and P. pratensis, P. sikkimensis and P. psilolepis showed much farther, with coefficient 10.5. This is similar to morphological taxonomy result, so RAPD is a useful supplementary method in assessing genetic relationship among Poa species.
出处
《草业学报》
CSCD
2002年第4期94-99,共6页
Acta Prataculturae Sinica
基金
国家 8 63项目 (2 0 0 1 AA2 4 4 0 81 )
甘肃省列项目 (QS- 981 - B31 - 0 2 4 )资助。
