摘要
本文继续观察感染后6～24个月的细粒棘球蚴在NIH小鼠体内发育的组织学及组织化学变化。结果表明,在鼠体内出现育囊的时间为感染后7～8个月,囊液内见到游离原头节及子囊的时间分别为8及10个月,并发现细粒棘蚴体内的糖原、DNA、RNA、碱性蛋白质的含量,AKP、ACP及ATP酶的活力,均以生发层的芽状突起部分及原头节内的较丰富和较强。
Further studies on histological and histochemical changes in developmental Echinococcus granulosus cyst have been carried out. Female NIH mice, each inoculated intraperitoneally with 2000 sheep strain protoscolices were sacrificed at monthly or bimonthly intervals 6-24 months after inoculation for histological and histochemical observations.
The results are summarized as follows:
1. Histological observations: 6-7 months after inoculation, bud like protrusions and cyst like structure appeared in germinal layer increased. The fertile cysts and protoscolices grown directely in germinal layer were found on 8 months after infection. Both the free-protoscolices and daughter cysts in cyst fluid were detected at 9 and 10 months after inoculation respectively. 12 to 24 months later, the E. granulosus cysts were enlarged gradually. Besides the progressive thickness of the laminated layer and ectocyst, a few stalked protoscolices arising from the outer surface of fertile cysts were seen in 22 months post infection. The histological alterations of germinal layer were similar to those seen in 7-10 months of E. granulosus cyst after inoculation.
2. Histochemical observations:-24 months after infection, the glycogen, DNA, RNA, alkaline protein, AKP,ACP and ATPase located mainly in bud like protrusions of germinal layer and protoscolex were more abundant and active than those detected in other parts of germinal layer.
出处
《地方病通报》
1992年第1期1-4,共4页
Endemic Diseases Bulletin
关键词
细粒棘球蚴
发育
组织学
组织化学
Echinococcus granulosus cyst
NIH mouse
Development
Histology
Histochemistry
