摘要
本文对细粒棘球绦虫原头蚴的组织化学成份进行了较为系统和全面的观察,结果表明,该虫体内存在糖原、RNA、DNA、蛋白质结合的α-氨基、酪氨酸、色氨酸及组氨酸、碱性蛋白质、酸性粘多糖、胶原及网状纤维成分、AKP、ATP酶及ACP等生化物质。并对上述成分的定位,含量及其生理学的意义进行了重点讨论。
The biochemical substances in protoscolex of Echinococcus granulosus were investigated systematically by histochemical techniques.
It was showed that glycogen, RNA, DNA, protein bound a-amino groups, tyrosine, tryptophan, histidine, alkaline protein, acid mucopolysaccharides, collagenous fibers, reticular fibers, activities of AKP, ACP and ATPase were detected at various levels in protoscoleccs of Echinococcus granulosus.
The content of glycogen was rich in parenchymal tissue of larva. RNA and DNA were mainly located in cytoplasm and cell nucleus, respectively. The content of protein bound α-amino groups, tyrosine, tryptophan, histidine and alkaline protein were very rich in tegument and small parenchymal cells. Both the activities of AKP and ATPase were very strong in tegument of protoscoleces. The hooks were contained heavy acid mucopolysaccharides and reticular fiber element and moderate contents of protein bound tyrosine, tryptophau an4 histidine. The collagenous fibers were noly presented in parenchymal tissue of larva. Some content of PAS positive material, RNA, DNA, protein bound α-amino groups, tyrosine, tryptophan and histidine, alkaline protein, acid muco-polysaccharides, collagenous and reticular fiber element, AKP, ACP and ATP-asc were found in calcareous corpuscles of protoscoleces.
The physiological significance of biochemical substances presented in proto-scoleces. obtained from sheep was discussed briefly.
关键词
细粒棘球绦虫
原头蚴
钙小体
糖原
Echinococcus granulosus, protoscolex, calcareous corpuscle, glycogen, nuelear acid, protein, AKP.
