摘要
目的 探讨基质金属蛋白酶 2 (matrixmetalloproteinase2 ,MMP 2 )的表达与侵袭性垂体腺瘤相关性研究 .以寻找垂体腺瘤侵袭性可能的发病机制 .方法 用免疫组化的方法对 54例垂体瘤患者的组织标本进行研究 .并对其中 1 6例患者采用逆转录 聚合酶链反应 (RT PCR)的方法对MMP 2mRNA的表达进行研究 .免疫组化结果用半定量的方法进行分析 .结果 54例垂体瘤患者中 ,1 2例为侵袭性垂体腺瘤 ,4 2例为非侵袭性垂体腺瘤 .免疫组化显示 ,侵袭性垂体腺瘤的MMP 2的表达明显高于非侵袭性垂体腺瘤 (分别为 3 9± 1 6 ,2 3± 1 4 ,P <0 0 0 1 ) .大型垂体腺瘤和微垂体腺瘤 ,以及功能性和非功能性垂体腺瘤的MMP 2的表达则无明显差别 .MMP 2的表达与Ki 6 7的表达无明显相关性 (r=- 0 0 5;P >0 0 5) .侵袭性垂体腺瘤MMP 2mRNA的表达 ,明显高于非侵袭性垂体腺瘤 (P <0 0 5) .结论 MMP 2的高表达与肿瘤的侵袭性密切相关 ,但与肿瘤的大小及分泌功能无明显关系 .MMP 2可以作为肿瘤侵袭性一项有效的指标 .
AIM The aim of this study is to evaluate the relationship between the expression of matrix metalloproteinase 2 (MMP 2) and the cavernous invasion of pituitary adenomas. METHOD Tissue samples from 54 pituitary adenomas were examined expression of MMP 2 protein by immunohistochemical method. In sixteen cases, the expression of MMP 2 mRNA were also examined by RT PCR assay. Immunostaining was semiquantatively scored based on intensity (0 3) and distribution (0 3). RESULTS Thirty fourth patients. There were 12 cases with cavernous invasion, and 42 were noninvasive cases. The MMP 2 score of pituitary adenoma patients with cavernous invasion (3 9±1 6) was significantly higher than those without invasion (2 3±1 4, P <0 001 ). There was no correlation between macroadenomas and micoadenomas, and no correlation between the functional adenomas and non functional adenomas. We didn't find positive correlation between the MMP 2 and Ki 67 labeling index ( r =-0 05, P >0 05). MMP 2 mRNA expression was also observed significantly higher in invasive pituitary adenomas than those without invasion ( P <0 01). CONCLUSION The study suggested that MMP 2 might be associated with the aggressiveness and invasion. MMP 2 expression is not related with the tumor size and secretion. MMP 2 is a useful tool for assessing the invasion.
出处
《第四军医大学学报》
北大核心
2002年第23期2185-2188,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (39970 752 )
