摘要
白藜芦醇(resveratrol,RSV)作为一种预防多种疾病的化合物,在延缓神经退行性疾病、抗氧化应激、抗炎症、抗癌等方面的药用价值已被广泛报道。给家蚕添食RSV,调查家蚕幼虫时期体重,观察中肠组织病理切片,并检测氧化应激指示物变化,结果发现添食RSV可以增加家蚕体重,对中肠组织病理学影响较小,可提升家蚕体内过氧化氢酶(catalase,CAT)活性、还原型谷胱甘肽(reduced glutathione,GSH)含量和超氧化物歧化酶(superoxide dismutase,SOD)活性,降低丙二醛(malondialdehyde,MDA)的积累。通过转录组学以及RT-qPCR验证,发现家蚕磷酸烯醇式羧化激酶(Bombyx mori phosphoenolpyruvate carboxykinase)基因BmPEPCK在RSV处理组AMPK、FoxO以及胰岛素等多条信号通路中均显著上调表达。采用RNAi技术干扰BmPEPCK基因的表达,检测家蚕体内氧化应激指示物变化,结果表明BmPEPCK基因表达量下降会引起家蚕氧化应激水平下降,还会导致家蚕体重降低。研究结果有助于进一步了解RSV在家蚕模型系统中氧化应激调节的机制,为抗氧化研究提供新的靶点,以延缓氧化应激相关疾病的发展。
Resveratrol(RSV),as a compound with potential for preventing multiple diseases,has been extensively reported for its medicinal value in neurodegenerative diseases,oxidative stress,inflammation,and cancer.Following RSV supplementation in domestic silkworms,investigations into larval body weight,examination of midgut histopathological sections,and measurement of oxidative stress markers revealed that RSV enhances silkworm body weight with minimal impact on midgut histopathology.It increased the catalase(CAT)activity,the reduced glutathione(GSH)content,and the superoxide dismutase(SOD)activity within silkworms while decreasing the malondialdehyde(MDA)accumulation.Transcriptomic analysis and RT-qPCR validation revealed that the Bombyx mori phosphoenolpyruvate carboxykinase gene BmPEPCK exhibited significantly upregulated expression in multiple signaling pathways,including AMPK,FoxO,and insulin,within the RSV-treated group.By using RNAi technology to interfere with the expression of BmPEPCK gene and detecting changes in oxidative stress indicators in silkworms,the results indicated that reduced BmPEPCK gene expression led to decreased oxidative stress levels in silkworms and also caused a reduction in silkworm body weight.These findings contribute to understanding the mechanisms of oxidative stress regulation by RSV in the silkworm model system,providing novel targets for antioxidant research to delay the progression of oxidative stress-related diseases.
作者
丁蕾
谷同玉
Vivian Andoh
张佳鑫
马爱琴
陈亮
Ding Lei;Gu Tongyu;Vivian Andoh;Zhang Jiaxin;Ma Aiqin;Chen Liang(School of Life Sciences,Jiangsu University,Zhenjiang Jiangsu 212013,China;Qingdao Vland Animal Health Group Co.,Ltd.,Qingdao Shandong 266100,China)
出处
《蚕业科学》
北大核心
2025年第6期513-522,共10页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金项目(31802140)
江苏大学人才科研促进基金项目(14JDG157)。
