摘要
脂肪酶催化制备改性磷脂的工艺具有良好的应用前景,游离脂肪酶水解效果差,因此交联酶聚集体(CLEAs)技术的发展对提高脂肪酶的水解效果具有重要意义。以戊二醛(Glu)为交联剂制备根霉脂肪酶的聚集体(Glu-CLEAs),并将其用于正己烷/水两相体系中催化磷脂水解反应。实验结果显示:优化Glu-CLEAs的制备条件后,其酶活力回收率为(99.72±2.34)%,红外结构表征和酶学性能检测显示Glu-CLEAs的结构刚性和稳定性增强。通过单因素实验筛选出Glu-CLEAs的最佳反应条件:体系水质量分数为20%、磷脂底物质量分数为30%、Glu-CLEAs质量分数为22%(1 675 U/mL)、催化反应8 h,最高水解率为(95.55±0.13)%。交联酶聚集体技术为脂肪酶催化磷脂制备改性磷脂提供了一条可行的技术路线。
The preparation of modified phospholipids through lipase catalysis holds promising application prospects.Given the limited hydrolysis efficiency of free lipase,the advancement of cross-linked enzyme aggregates(CLEAs)technology is crucial for enhancing the hydrolysis efficiency of lipase.In this study,Rhizopus lipase aggregates(Glu-CLEAs)were prepared using glutaraldehyde(Glu)as a crosslinking agent and utilized as catalysts for phospholipid hydrolysis in an n-hexane/water biphasic system.The results showed that,after optimizing the preparation conditions of Glu-CLEAs,its enzyme activity recovery rate reached(99.72±2.34)%.Infrared spectroscopy and enzymatic performance assays demonstrated improved structural rigidity and enhanced stability of Glu-CLEAs.Under optimized conditions of 20%water content,30%substrate concentration,22%Glu-CLEAs concentration(1675 U/mL),and 8-hour catalytic reaction,the catalytic efficiency reached(95.55±0.13)%,as determined through single-factor experiments.These findings indicated that the CLEAs technology provides an effective approach for the lipase-catalyzed preparation of modified phospholipids.
作者
钱俊青
顾国秀
赵晓华
QIAN Junqing;GU Guoxiu;ZHAO Xiaohua(College of Pharmaceutical Science,Zhejiang University of Technology,Hangzhou 310014,China)
出处
《浙江工业大学学报》
北大核心
2026年第2期201-207,共7页
Journal of Zhejiang University of Technology
基金
国家“十三五”重点研发计划项目子课题(2016YFD0400803)。
关键词
改性磷脂
酶法催化
脂肪酶
交联酶聚集体
modified phospholipids
enzymatic catalysis
lipase
cross-linking enzyme aggregate
