摘要
目的：制备松萝酸磷脂复合物并对其进行表征。方法：采用溶剂反应法制备松萝酸磷脂复合物,以复合率和松萝酸质量分数为指标,采用单因素试验和正交试验优选该复合物的制备工艺参数;对该复合物的油水分配系数进行测定,利用紫外光谱法、傅里叶变换红外光谱法、体式显微镜、扫描电镜对复合物结构进行表征。结果：松萝酸磷脂复合物的最佳制备工艺条件为松萝酸质量浓度10 g·L（-1）,松萝酸-磷脂（1∶1）,反应时间5 h,反应温度50℃;松萝酸磷脂复合物的复合率86.85%,松萝酸质量分数38.46%,油水分配系数的对数值2.35。紫外光谱法、体式显微镜等结果表明松萝酸磷脂复合物已形成。结论：优化的制备工艺稳定可行,可为松萝酸的进一步研究与开发提供参考。
Objective： The usnic acid phospholipid complex was prepared and characterized. Method：The complex was prepared by solvent reaction method. Taking recombination rate and content of usnic acid as index,the preparation process parameters of the complex were optimized by single factor tests and orthogonal test.The oil/water partition coefficient of this complex was determined and the structure of the complex was characterized by ultraviolet spectroscopy（ UV）,Fourier transform infrared spectroscopy（ FT-IR）,stereomicroscope and scanning electron microscope（ SEM）. Result： The optimal preparation conditions were as follows： the drug concentration of10 g·L^（-1）,usnic acid-phospholipid（ 1 ∶ 1）,reaction time of 5 h and reaction temperature of 50 ℃. The recombination rate of the complex prepared under these optimized conditions was 86. 85%,and the content of usnic acid was 38. 46%. The logarithm value of oil/water partition coefficient was 2. 35. UV,FT-IR,stereomicroscope and SEM analysis showed that the complex had been formed. Conclusion： This optimized preparation technology in this research is stable and feasible,it can provide conditions for further study of usnic acid.
作者
赵颖
陈剑
武小赟
郭亚飞
宋婷
李智豪
宋丹
管海燕
ZHAO Ying;CHEN Jian;WU Xiao-yun;GUO Ya-fei;SONG Ting;LI Zhi-hao;SONG Dan;GUAN Hai-yan(School of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China;Chongqing Technical Center for Drug Evaluation ＆ Certification,Chongqing 401120,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2018年第21期20-25,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
重庆市科委基础与前沿研究计划项目(cstc2014jcyj A10037)
重庆市教委科学技术研究项目(KJ1709218)
关键词
松萝酸
磷脂复合物
正交试验
表征
紫外光谱法
复合率
油水分配系数
usnic acid
phospholipid complex
orthogonal test
characterization
ultraviolet spectroscopy
recombination rate
oil/water partition coefficient
