摘要
目的评价不同产地红花药材的质量。方法采用高效液相色谱法测定药材样品中羟基红花黄色素A、山柰酚含量,采用紫外分光光度法测定样品中总黄酮含量,采用原子吸收分光光度法测定样品中铅(Pb)、镉(Cd)、砷(As)、汞(Hg)、铜(Cu)含量,采用气相色谱串联质谱法测定样品中33种禁用农药残留量,采用主成分分析法分析上述3种活性成分的贡献度。结果57批红花药材样品中羟基红花黄色素A、山柰酚、总黄酮的含量分别为0.80%~2.60%、0.026%~0.239%、4.10%~10.05%。共提取了2个主成分,累计方差贡献率为88.20%,载荷均较高的成分为羟基红花黄色素A。仅产自云南省大理白族自治州巍山县牛街镇的样品中Hg元素超标(>0.2 mg/kg)。抽检的15批样品中均未检出33种禁用农药。结论不同产地红花药材中羟基红花黄色素A、山柰酚、总黄酮的含量存在差异,且整体重金属、有害元素及农药残留的安全风险均较低,建立的方法可用于评价不同产地红花药材的质量。
Objective To evaluate the quality of Carthami Flos from different habitats.Methods High-performance liquid chromatography(HPLC)method was used for the content determination of hydroxysafflor yellow A,and kaempferol in samples.UV spectrophotometry was used for the content determination of total flavonoids in samples.Atomic absorption spectrophotometry was used for the content determination of lead(Pb),cadmium(Cd),arsenic(As),mercury(Hg),and copper(Cu)in samples.Gas chromatography-tandem mass spectrometry was used to determine the residue of 33 banned pesticides in samples.Principal component analysis was used to analyze the contribution of hydroxysafflor yellow A,kaempferol,and total flavonoids.Results The contents of hydroxysafflor yellow A,kaempferol and total flavonoids in 57 batches of Carthami Flos samples were in the ranges of 0.80%-2.60%,0.026%-0.239%,and 4.10%-10.05%,respectively.Two principal components were extracted with a cumulative variance contribution rate of 88.20%.The component with high loadings was hydroxysafflor yellow A.The Hg content in samples only collected from Niujie Town,Weishan County,Dali Bai Autonomous Prefecture,Yunnan Province exceeded the standard requirements,and 33 kinds of prohibited pesticides were not detected in the 15 batches of samples.Conclusion The contents of hydroxysafflor yellow A,kaempferol and total flavonoids in Carthami Flos from different habitats were different,and the overall safety risks of heavy metals harmful elements,and pesticide residues are low.The established method can be used to evaluate the quality of Carthami Flos from different habitats.
作者
王沛琦
胡尊红
杨博
杨谨
单奎
段彦君
张文艳
陈西垚
王硕
胡学礼
WANG Peiqi;HU Zunhong;YANG Bo;YANG Jin;SHAN Kui;DUAN Yanjun;ZHANG Wenyan;CHEN Xiyao;WANG Shuo;HU Xueli(Industrial Crop Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,Yunnan 650205,China;Nanjian County Branch of Dali Tobacco Company,Dali,Yunnan 671099,China;Institute of Medicinal Plants and New Agricultural Technology,Dali Academy of Agricultural Sciences Popularization,Dali,Yunnan 671600,China;Nanjian Yi Autonomous County Agricultural Technology Extension Center,Dali,Yunnan 671099,China;Baoshan Agricultural Technology Extension Center,Baoshan,Yunnan 678000,China)
出处
《中国药业》
2026年第7期85-89,共5页
China Pharmaceuticals
基金
云南省科学技术厅科技计划项目[202304BI090020,202402AA310008]
云南省烟草公司大理州公司科技计划一般项目。
关键词
红花
羟基红花黄色素A
山柰酚
总黄酮
重金属
有害元素
农药残留量
质量评价
Carthami Flos
hydroxysafflower yellow A
kaempferol
total flavonoids
heavy metals
harmful elements
pesticide residue levels
quality evaluation
