摘要
建立鸡蛋中叶黄素、斑蝥黄、β-阿朴-8’-胡萝卜素醛、β-阿朴-8’-胡萝卜素酸乙酯、β-胡萝卜素5种饲用着色剂同时检测的固相萃取-高效液相色谱法(high performance liquid chromatography,HPLC)。样品经二氯甲烷-乙腈(20∶80,V/V)提取,Silica固相萃取柱净化,采用C_(30)色谱柱(250 mm×4.6 mm,5μm)分离,用甲醇-二氯甲烷(85∶15,V/V)进行梯度洗脱,结合高效液相色谱法完成测定,并通过外标法实现定量分析。结果表明,5种饲用着色剂在0.05~50μg·mL^(-1)质量浓度范围线性关系良好,相关系数r^(2)均大于0.9992,方法检出限在0.01~0.20 mg·kg^(-1)之间,定量限在0.03~0.67 mg·kg^(-1)之间。当加标水平分别设定为0.1、1、5 mg·kg^(-1)时,5种饲用着色剂的平均回收率在87.42%~95.07%之间,RSD≤3.0%。该方法提取效果好,适用于鸡蛋中5种饲用着色剂同时测定。
Asolid phase extraction-HPLC method for simultaneous determination of lutein,canthaxanthin,β-Apo-8’-carotenoicaldehyde,β-Apo-8’-carotenoic ethylester,β-carotene in eggs was established.The samples were extracted by dichloromethane-acetonitrile(20∶80,V/V)and purified by silica solid phase extraction column.The analytes were separated by C_(30) column(250 mm×4.6 mm,5μm)with methanol-dichloromethane(85∶15,V/V)as mobile phases in gradient model.Then the analytes were detected with HPLC.Quantitative by external standard method.The results showed that the 5 kinds of feedstuff colorants had a good linear relationship in the concentration range of 0.05~50μg·mL^(-1),and the correlation coefficient were all above 0.9992.The limits of detection of the method were between 0.01 and 0.20 mg/kg,with the limits of quantitation were between 0.03 and 0.67 mg·kg^(-1).At the spiked levels of 0.1,1 and 5 mg·kg^(-1),the recoveries of eggs samples were 87.42%~95.07%,respectively,with RSD≤3.0%.This method had a good extraction effect,and was suitable for the quantitative analysis of 5 kinds of feedstuff colorants in eggs.
作者
禹洁
周佳
苏阿龙
王小花
王丽君
尹娟娟
YU Jie;ZHOU Jia;SU Along;WANG Xiaohua;WANG Lijun;YIN Juanjuan(Gansu Food Inspection and Research Institute,Lanzhou 730300,China)
出处
《现代食品》
2026年第2期186-192,共7页
Modern Food
基金
甘肃省市场监督管理局科技计划项目(SSCJG-SP-B202204)。
关键词
高效液相色谱法
饲用着色剂
鸡蛋
high performance liquid chromatography
feedstuff colorants
egg
