摘要
目的 探讨在血液标本丙肝病毒检测中应用核酸检测法与酶联免疫吸附法(ELISA)的效果。方法 选取贵州省血液中心2023年5月至2024年5月送检的425份血液样本,均开展丙肝病毒核糖核酸(HCV RNA)检测,并将检测结果作为金标准。对所有血液标本均进行核酸检测、ELISA检测,比较两种检测方法单独与联合检测的结果、诊断效能。结果 425份血液样本,经HCV RNA检测,有阳性33份;经ELISA检测,有阳性22份;经核酸检测,有阳性24份;两者联合检测,有阳性32份。两者联合检测的准确率、特异度分别为98.35%、98.47%,敏感度为96.97%,阳性、阴性预测值分别为84.21%、99.74%,均高于两者单一检测的结果,P<0.05。结论 在血液标本丙肝病毒检测中联合应用核酸检测法与ELISA,有助于提高疾病筛查率,指导后续治疗。
Objective:To explore the effects of nucleic acid detection and enzyme-linked immunosorbent assay(ELISA)in the detection of hepatitis C virus in blood samples.Methods:425blood samples from Guizhou Blood Center from May 2023to May 2024were tested for HCV RNA,and the test results were regarded as the gold standard.All blood samples were detected by nucleic acid and ELISA,and the results and diagnostic efficiency of the two detection methods were compared.Results:Among 425blood samples,33were positive by HCV RNA detection.22samples were positive by ELISA;24samples were positive by nucleic acid detection;32samples were positive by combined detection of the two methods.The accuracy,specificity and sensitivity of the combined detection were 98.35%and 98.47%、96.97%,and the positive and negative predictive values were 84.21%and 99.74%,respectively,which were higher than those of the single detection,P<0.05.Conclusion:The combined application of nucleic acid detection and ELISA in the detection of hepatitis C virus in blood samples is helpful to improve the screening rate of diseases and guide the follow-up treatment.
作者
蔡健
余芳
吴国将
Cai Jian;Yu Fang;Wu Guojiang(Laboratory of Guizhou Blood Center,Guiyang Guizhou 550001;Department of Composition,Guizhou Blood Center,Guiyang Guizhou 550001,China)
出处
《生命科学仪器》
2025年第5期45-47,共3页
Life Science Instruments
关键词
血液标本
丙肝病毒
核酸检测法
ELISA
Blood specimen
Hepatitis C virus
Nucleic acid detection method
ELISA
