摘要
针对养殖水体中存在的氮及抗生素污染等问题,采用小麦秸秆为原料制备生物碳基载体,以筛选分离出的氮及四环素(TC)高效降解菌株W3为固定化菌种,采用吸附法制备生物炭基固定化菌剂,并优化其制备参数(生物炭投加量、接种量和固定化时间)。研究结果表明,该生物炭具有介孔结构及丰富的-COOH、-OH、-C=C-OH等官能团,有利于微生物细胞的黏附与增殖。菌株W3经鉴定为脱氮副球菌(Paracoccus denitrificans),该菌株在低溶解氧条件下对氮及TC的降解效果较好。当生物炭投加量为4 mg/mL、菌剂接种量为40%、固定化时间为12 h时制备得到的固定化微生物菌剂对硝酸盐氮(NO_(3)^(-)-N)、亚硝酸盐氮(NO_(2)^(-)-N)和TC具有最佳的降解效果,24 h后NO_(3)^(-)-N和TC的降解率分别为99.66%和90.50%。
In response to the nitrogen and antibiotic pollution in aquaculture water,wheat straw is used as raw material to prepare biochar based carriers,and the highly efficient nitrogen and tetracycline(TC)degrading strain W3 is isolated as the immobilized strain.The biochar based immobilized bacterial agent is prepared by adsorption method.The optimal preparation conditions for the biochar based immobilized bacterial agent are investigated(biochar dosages,inoculation amounts,and immobilization times).The results showed that the biochar based carrier have a mesoporous structure and abundant functional groups such as-COOH,-OH,-C=C-OH,etc.,which have good affinity for microbial cell membranes and are conducive to the adhesion and proliferation of microbial cells.Strain W3 has been identified as Paracoccus denitrificans,which has a good degradation effect on nitrogen and TC under low dissolved oxygen conditions.When the dosage of biochar is 4 mg/mL,the inoculation amount of microbial agent is 40%,and the immobilization time is 12 h,the immobilized microbial agent prepared has the best degradation effect on nitrate(NO_(3)^(-)-N),nitrite(NO_(2-)^(-)N),and TC.After 24 h,the degradation rates of NO_(3)^(-)-N and TC are 99.66%and 90.50%,respectively.The preparation of biochar based immobilized microbial agents is beneficial for the resource utilization of straw and provides bioremediation materials for solving nitrogen and TC pollution problems in aquaculture water.
作者
刘志均
王翔成
薛江鹏
韦良焕
徐文杰
LIU Zhijun;WANG Xiangcheng;XUE Jiangpeng;WEI Lianghuan;XU Wenjie(School of Environmental Engineering,Nanjing Institute of Technology,Nanjing 211167,China;Xinjiang Biomass Solid Waste Resources Technology and Engineering Center,Kashi University,Kashi 844000,China)
出处
《水处理技术》
北大核心
2026年第3期87-92,共6页
Technology of Water Treatment
基金
江苏省大学生创新创业训练计划项目(202311276096Y)
新疆生物类固废资源化工程技术研究中心开放课题(KSUGCZX202304)
江苏省产学研合作项目(BY20230601)
南京工程学院青年基金项目(QKJ202203)。
关键词
生物炭
硝酸盐
抗生素
固定化微生物
biochar
nitrate
antibiotics
immobilized microorganisms
