摘要
目的 系统鉴定竹节参的主要化学成分,并研究润制时间、蒸制时间及烘干温度对其炮制品(熟竹节参)中有效成分人参皂苷Rg1含量的影响,为竹节参的质量控制及炮制工艺优化提供科学依据。方法 采用液相色谱-静电场轨道阱高分辨质谱(LC-Orbitrap-MS)技术,结合mzCloud与mzVault数据库,对竹节参的主要化学成分进行定性分析;通过设定不同润制时间、蒸制时间及烘干温度制备熟竹节参样品;样品经乙醚回流提取,回收乙醚后以甲醇加热处理至溶液无色,再经饱和正丁醇萃取,并以氨试液制备供试品溶液;采用高效液相色谱法(HPLC)测定不同炮制工艺条件下人参皂苷Rg1的含量,从而优选最佳炮制工艺。结果 在润制3 h、蒸制6 h、干燥温度90℃的炮制条件下,熟竹节参中人参皂苷Rg1含量达到最高;通过LC-Orbitrap-MS分析,共鉴定出31个化学成分,结构类型涵盖达玛烷型、奥寇梯木烷型及齐墩果烷型等。结论 本研究确定了以润制3 h、蒸制6 h、90℃干燥为核心的最佳炮制工艺,该工艺可显著提高熟竹节参中人参皂苷Rg1的含量。所建立的LC-Orbitrap-MS方法稳定、可靠,可用于竹节参的质量控制及综合开发利用。
Objective To systematically identify the main chemical components of Zhujieshen(Panacis Japonici Rhizoma)and investigate the effects of moistening time,steaming time,and drying temperature on the content of the active ingredient ginsenoside Rg1 in its processed products[processed Zhujieshen(Panacis Japonici Rhizoma)],providing a scientific basis for quality control and processing technology optimization of Zhujieshen(Panacis Japonici Rhizoma).Methods Liquid chromatography-electrostatic field orbitrap high-resolution mass spectrometry(LC-Orbitrap-MS)technology,combined with the mzCloud and mzVault databases,was employed for the qualitative analysis of the main chemical components of Zhujieshen(Panacis Japonici Rhizoma).Processed Zhujieshen(Panacis Japonici Rhizoma)samples were prepared by setting different moistening times,steaming times,and drying temperatures.The samples were subjected to reflux extraction with diethyl ether.After recovering the diethyl ether,the samples were heated with methanol until the solution became colorless,followed by extraction with saturated n-butanol and preparation of the test sample solution with ammonia test solution.High-performance liquid chromatography(HPLC)was used to determine the content of ginsenoside Rg1 under different processing conditions,thereby optimizing the best processing technology.Results Under the processing conditions of 3 h of moistening,6 h of steaming,and a drying temperature of 90℃,the content of ginsenoside Rg1 in processed Zhujieshen(Panacis Japonici Rhizoma)reached the highest level.Through LC-Orbitrap-MS analysis,a total of 31 chemical components were identified,with structural types covering dammarane,ocotillol,and oleanane types,among others.Conclusion This study determined the optimal processing technology centered on 3 h of moistening,6 h of steaming,and drying at 90℃,which could significantly increase the content of ginsenoside Rg1 in processed Zhujieshen(Panacis Japonici Rhizoma).The established LC-Orbitrap-MS method is stable and reliable and can be used for the quality control and comprehensive development and utilization of Zhujieshen(Panacis Japonici Rhizoma).
作者
冷兴旺
李悦
刘吉生
罗江溢
梁玲
邱伊星
彭彩云
袁汉文
LENG Xingwang;LI Yue;LIU Jisheng;LUO Jiangyi;LIANG Ling;QIU Yixing;PENG Caiyun;YUAN Hanwen(Chinese Medicine and Ethnomedicine Innovation&Development International Laboratory,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)
基金
国家自然科学基金项目(82405012)。
