摘要
本文探究饲料中硒对赤拟谷盗(Tribolium castaneum)生长发育、种群动态、氧化应激反应及多杀菌素耐受性的影响。结果表明,硒处理显著延长了一龄幼虫的发育历期,但缩短了五龄幼虫历期;低硒饲养赤拟谷盗的内禀增长率(r)、周限增长率(λ)和净增殖率(R_(0))及成虫存活率均显著高于高硒组;高硒处理导致赤拟谷盗成虫存活率和各年龄阶段期望寿命显著下降。氧化应激方面,幼虫的超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性普遍高于成虫,而过氧化物酶(POD)活性则相反。与对照组相比,硒处理显著升高幼虫SOD活性但降低成虫SOD活性。幼虫CAT活性在硒处理下整体升高(低硒组仍低于对照组);而成虫CAT活性在所有硒处理中均受到抑制。谷胱甘肽S-转移酶(GST)活性在幼虫和成虫中均被硒显著诱导,表明硒对昆虫抗氧化酶活性具有显著影响,且受到虫态差异性和剂量效应的影响。同时硒处理导致幼虫中脂质过氧化产物丙二醛(MDA)含量显著上升,而成虫中,低硒组MDA含量则显著降低,高硒组与对照组无差异。与对照组相比低硒组成虫对多杀菌素耐受性最强,高硒组次之。硒可能通过调节赤拟谷盗的氧化还原稳态,进而影响其生长发育、种群增长及杀虫剂耐受性,并且这种影响因浓度、虫态而异。
In this study,the effects of selenium in diets on the growth and development,population dynamics,oxidative stress response,and spinosad tolerance of Tribolium castaneum were investigated.The results showed that the development duration of the first-instar larvae was significantly prolonged,while that of the fifth-instar larvae was significantly shortened by selenium treatment.The intrinsic rate of increase(r),finite rate of increase(λ),net reproductive rate(R_(0))and adult survival rate of T.castaneum reared on low-selenium diets were significantly higher than those reared on high-selenium diets.High-selenium treatment resulted in a significant decrease in the adult survival rate and age-specific life expectancy of T.castaneum adults at all developmental ages.In terms of oxidative stress,the activities of superoxide dismutase(SOD)and catalase(CAT)in larvae were generally higher than those in adults,whereas the activity of peroxidase(POD)showed the opposite trend.Compared with the control group,the SOD activity of larvae was significantly increased,while that in adults was significantly decreased by selenium treatment.The CAT activity in larvae was generally increased under selenium treatment(although it remained lower than that in the control group),whereas the CAT activity in adults was significantly inhibited in all selenium-trated groups.The activity of glutathione S-transferase(GST)was significantly induced by selenium in both larvae and adults,indicating that selenium exterted a significant effect on the activities of antioxidant enzymes in insects,which was affected by the developmental stage differences and dose-dependent effects.At the same time,the content of malondialdehyde(MDA),a lipid peroxidation product,in larvae was significantly increased by selenium treatment.In adults,however,the MDA content in the low-selenium group was significantly reduced,while no significant difference was observed between the high-selenium group and the control group.In addition,compared with the control group,the adults in the low-selenium group exhibited the strongest tolerance to spinosad,followed by those in the high-selenium group.In summary,selenium may affect the growth and development,population growth,and insecticide tolerance of T.castaneum by regulating its redox homeostasis,and such effects varied with the selenium concentration and insect developmental stage.
作者
李嘉旭
程思宇
邓婷文
刘钰
郭璇
汤秋玲
毕洁
贺艳萍
Li Jiaxu;Cheng Siyu;Deng Tingwen;Liu Yu;Guo Xuan;Tang Qiuling;Bi Jie;He Yanping(School of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023;Key Laboratory for Deep Processing of Major Grain and Oil,Ministry of Education,Wuhan Polytechnic University,Wuhan 430023)
出处
《中国粮油学报》
北大核心
2026年第1期179-186,共8页
Journal of the Chinese Cereals and Oils Association
关键词
硒
赤拟谷盗
发育历期
氧化应激
耐药性
selenium
Tribolium castaneum
developmental duration
oxidative stress
insecticide tolerance
