摘要
目的建立一种冷凝收集-离子色谱方法,测定人体呼出气中的葡萄糖、蔗糖和果糖。方法通过自制的呼气冷凝装置对人体呼出气体进行冷凝和收集,采用离子色谱-脉冲安培法对人体呼出气冷凝液(EBC)中的葡萄糖、蔗糖和果糖进行分析。建立标准的EBC采集方法,并对采集过程中的冷却温度、采样流速等关键因素进行优化。同时采集健康受试者和糖尿病患者的实际EBC样品进行检测。结果健康受试者空腹时呼出气中只检出葡萄糖,含量为0.11~0.36 ng/L。健康志愿者进食后1 h、2 h、4 h的葡萄糖、蔗糖和果糖的含量分别为0.15~0.86、0~1.17和0~0.31 ng/L。糖尿病患者餐后1 h呼出气中的葡萄糖、蔗糖和果糖含量水平比健康受试者高10~100倍。结论该方法无创检测,可重复性强,可作为测定人体呼出气中葡萄糖、蔗糖和果糖的检测方法。
Objective To develop a method for the determination of glucose,sucrose and fructose in human exhaled breath by non-invasive condensation collection coupled with ion chromatography.Methods A self-designed exhaled breath condensation device was constructed,through which human exhaled breath was condensed and collected.The glucose,sucrose and fructose in collected human exhaled breath condensate(EBC)was analyzed by ion chromatography with a pulsed amperometric detection.The standard EBC collection method was established,and the key factors such as cooling temperature and sampling flow rate during condensation collection were investigated.Further more,EBC from healthy people and diabetic patients were collected.Results In healthy volunteers,only glucose was detected in the breath at fasting,with a concentration of 0.11 to 0.36 ng/L.The breath glucose,sucrose and fructose level in healthy volunteers after eating at 1 h,2 h and 4 h were 0.15-0.86,0-1.17 and 0-0.31 ng/L respectively.The concentration of glucose,sucrose and fructose in human exhaled breath of diabetic patients 1 h after a meal were 10-100 times higher than that of healthy subjects.Conclusion This method is non-invasive,reproducible.It can be used as a detection method for measuring glucose,sucrose and fructose in human exhaled breath.
作者
倪承珠
陈佳萍
周杨叶
朱岩
甘露
卓延红
NI Chengzhu;CHEN Jiaping;ZHOU Yangye;ZHU Yan;GAN Lu;ZHUO Yanhong(Physical and Chemical Testing Institute,Taizhou Center for Disease Control and Prevention(Taizhou Health Supervision Institute),Taizhou,Zhejiang 318000,China)
出处
《环境与健康杂志》
2026年第1期77-82,共6页
Journal of Environment and Health
基金
浙江省自然科学基金(LTGC24B050010)
台州市高层次人才计划“特殊支持”人才(台卫发[2025]-13号)。
关键词
呼出气冷凝收集
离子色谱-脉冲安培检测
葡萄糖
蔗糖
果糖
Exhaled breath condensate(EBC)
Ion chromatography-pulsed amperometric detection
Glucose
Fructose
Sucrose
