摘要
本试验旨在研究白蒺藜副产物对滩羊瘤胃发酵参数和瘤胃菌群结构的影响。选择体重(24.68±0.90)kg的健康3月龄滩羊公羔68只,编号后随机分为4组,每组17只,单栏饲养。4组羔羊分别饲喂添加0(对照组)、5%(试验Ⅰ组)、10%(试验Ⅱ组)和15%(试验Ⅲ组)白蒺藜副产物的试验饲粮。预试期10 d,正试期60 d。结果显示:1)与对照组相比,试验Ⅰ组的丙酸摩尔比例极显著提高(P<0.01),试验Ⅰ组和试验Ⅱ组异丁酸与异戊酸摩尔比例分别极显著(P<0.01)和显著降低(P<0.05),试验Ⅰ组和试验Ⅲ组戊酸摩尔比例极显著降低(P<0.01),各试验组丁酸摩尔比例均极显著降低(P<0.01)。2)各组间瘤胃菌群Alpha多样性指数均差异不显著(P>0.05);主坐标分析(PCoA)和非度量多维尺度分析(NMDS)表明,试验Ⅰ组与对照组的瘤胃菌群结构类似。3)与对照组相比,试验Ⅰ组糖发酵菌属(Saccharofermentans)的相对丰度极显著升高(P<0.01);3个试验组瘤胃球菌属(Ruminococcus)的相对丰度均极显著降低(P<0.01)。4)KEGG通路富集分析显示,与对照组相比,试验Ⅰ组糖链生物合成与代谢、内分泌系统、萜类和聚酮化合物代谢和能量代谢的相对丰度显著升高(P<0.05)。综上所述,饲粮中添加5%的白蒺藜副产物对滩羊瘤胃发酵具有积极影响,可使瘤胃发酵模式向丙酸型转化,且不会对瘤胃菌群结构和多样性产生负面影响。
This experiment was conducted to investigate the effects of Tribulus terrestris by-products(TTB)on rumen fermentation parameters and rumen microflora structure of Tan sheep.Sixty-eight healthy 3-month-old male Tan sheep lambs with an average body weight of(24.68±0.90)kg were selected,numbered,and randomly divided into four groups(17 lambs per group),with individual pen feeding.The four groups were fed experimental diets supplemented with 0(control group),5%(trial groupⅠ),10%trial groupⅡ),and 15%(trial groupⅢ)TTB,respectively.The pre-trial period lasted for 10 days,followed by a 60-day formal trial period.The results showed that:1)compared with the control group,the molar ratio of propionate in trial groupⅠwas extremely significantly increased(P<0.01),the molar ratios of isobutyrate and isovalerate in trial groupsⅠandⅡwere extremely significantly(P<0.01)and significantly decreased(P<0.05),respectively),the molar ratio of valerate in experimental groupsⅠandⅢwas extremely significantly decreased(P<0.01),and the molar ratio of butyrate in all trial groups was extremely significantly decreased(P<0.01).2)No significant differences were observed in the Alpha diversity indexes of the rumen microflora among the groups(P>0.05).Principal coordinate analysis(PCoA)and non-metric multidimensional scaling analysis(NMDS)indicated that the rumen microflora structure of trial groupⅠwas similar to that of the control group.3)Compared with the control group,the relative abundance of Saccharofermentans in trial groupⅠwas extremely significantly increased(P<0.01),and the relative abundance of Ruminococcus in three trial groups was extremely significantly decreased(P<0.01).4)KEGG pathway enrichment analysis revealed that,compared with the control group,trial groupⅠexhibited significantly elevated relative abundances in pathways related to glycan biosynthesis and metabolism,endocrine systems,metabolism of terpenoids and polyketides,and energy metabolism(P<0.05).In conclusion,dietary supplementation with 5%TTB has a positive effect on rumen fermentation of Tan sheep,shifting the rumen fermentation pattern toward propionate production without negatively affecting the rumen microflora structure and diversity.
作者
李瑞珍
王泰露
杨宇为
马青
许瑞
周玉香
LI Ruizhen;WANG Tailu;YANG Yuwei;MA Qing;XU Rui;ZHOU Yuxiang(College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China;Institute of Animal Research,Ningxia Academy of Agriculture and Forestry,Yinchuan 750002,China;Chengguan Animal Husbandry and Veterinary Workstation,Pingluo 753400,China)
出处
《动物营养学报》
北大核心
2026年第2期1247-1264,共18页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
滩羊精准养殖关键技术研究与集成示范(2022BBF02020)。
关键词
白蒺藜副产物
滩羊
瘤胃发酵参数
瘤胃菌群
Tribulus terrestris by-products
Tan sheep
rumen fermentation parameters
rumen microflora
