摘要
【目的】探究饲粮中添加盐地碱蓬提取物(SSE)对湖羊瘤胃发酵参数及胃肠道微生物群落结构的影响。【方法】选取36只体重(30.27±1.18)kg健康湖羊公羔,随机分为对照组(CON)和试验组(SSE),每组6个重复,每个重复3只羊。对照组采用全混合日粮(TMR),试验组在TMR基础上添加0.6%SSE。预饲期15 d,正试期30 d。于正试期第30天空腹采集湖羊瘤胃液和直肠粪便样本,测定瘤胃发酵参数和瘤胃、直肠粪便微生物。【结果】(1)相较于CON组,SSE组湖羊瘤胃总挥发性脂肪酸(TVFA)含量极显著提高(P<0.01),瘤胃pH、乙酸、丙酸、异丁酸、异戊酸和戊酸含量均显著提高(P<0.05)。(2)两组湖羊瘤胃微生物在门、属水平上的组成及Alpha、Beta多样性指数均无显著差异(P>0.05),SSE组肠滴虫属的相对丰度显著高于CON组(P<0.05)。瘤胃微生物与瘤胃发酵参数的相关性分析显示,黏螺菌属的相对丰度与瘤胃丁酸浓度呈显著正相关(P<0.05),解琥珀酸菌属的相对丰度与瘤胃丁酸含量呈显著负相关(P<0.05)。(3)两组湖羊直肠粪便微生物在门水平上的组成及Alpha、Beta多样性指数均无显著差异(P>0.05);与CON组相比,SSE组湖羊直肠中普雷沃菌属、阿加托杆菌属、丁酸弧菌属A、尤杆菌属J和甲基杆菌属的相对丰度均显著提高(P<0.05)。【结论】饲粮中添加0.6%SSE可优化湖羊瘤胃发酵参数,改善瘤胃微生物群落结构与组成,提高肠道中阿加托杆菌属、丁酸弧菌属和尤杆菌属等有益菌的相对丰度。
【Objective】This experiment aimed to investigate the effects of dietary supplementation with Suaeda salsa extract(SSE)on rumen fermentation parameters and gastrointestinal(ruminal and intestinal)microbial community structure in Hu sheep.【Method】Thirty-six healthy male Hu sheep lambs with an average body weight of(30.27±1.18)kg were randomly divided into a control group(CON)and an experimental group(SSE),with 6 replicates per group and 3 lambs per replicate.The sheep in CON group were received a total mixed ration(TMR),while in SSE group were fed TMR supplemented with 0.6%SSE.The pre-test period lasted for 15 days,followed by a 30-day formal trial period.On the 30th day of the formal trial,rumen fluid and rectal faecal samples were collected from fasted Hu sheep to determine rumen fermentation parameters and ruminal faecal microbial communities.【Result】①Compared with CON group,the ruminal total volatile fatty acid(TVFA)content of sheep in SSE group was extremely significantly increased(P<0.01),and ruminal pH,acetate,propionate,isobutyrate,isovalerate and valerate contents were significantly increased(P<0.05).②No significant differences were found in ruminal microbial composition at either the phylum or genus level,nor in Alpha and Beta diversity indices between the two groups(P>0.05).However,the relative abundance of the genus Enteromonas was significantly higher in SSE group(P<0.05).Correlation analysis between ruminal microbes and fermentation parameters showed that the relative abundance of Limivicinus was significantly positively correlated with ruminal butyrate concentration(P<0.05),while the relative abundance of Succiniclasticum was significantly negatively correlated with ruminal butyrate content(P<0.05).③No significant differences in rectal faecal microbial composition at the phylum level,or in the Alpha and Beta diversity indices was observed between the two groups(P>0.05).Compared with control group,the relative abundance of Prevotella,Agathobacter,Butyrivibrio_A,Eubacterium_J,and Methylobacterium in the rectum of SSE group were significantly increased(P<0.05).【Conclusion】Dietary supplementation with 0.6%SSE to the diet could optimize rumen fermentation parameters,improve the structure and composition of ruminal microbial communities,and increase the genera Agathobacter,Butyricimonas,and Eubacterium in the intestine of Hu sheep.
作者
李昌昌
武莹莲
杨振祥
李林贵
秦荣艳
刘艳丰
许贵善
王文奇
刁其玉
LI Changchang;WU Yinglian;YANG Zhenxiang;LI Lingui;QIN Rongyan;LIU Yanfeng;XU Guishan;WANG Wenqi;DIAO Qiyu(Xinjiang Key Laboratory of Herbivorous Livestock Feed Biotechnology,Feed Research Institute,Xinjiang Academy of Animal Science,Urumqi 830011,China;Key Laboratory of Livestock and Grass Resources Utilization around Tarim(Ministry of Agriculture and Rural Affairs,Co-construction by Ministries and Provinces),School of Animal Science and Technology,Tarim University,Alar 843300,China;Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《中国畜牧兽医》
北大核心
2026年第2期749-760,共12页
China Animal Husbandry & Veterinary Medicine
基金
国家肉羊产业技术体系项目(CARS-38)
国家重点研发计划(2021YFD1600701-2)
新疆维吾尔自治区羊产业体系(XJARS-09-09)。
关键词
盐地碱蓬提取物
湖羊
瘤胃发酵
瘤胃微生物
肠道微生物
Suaeda salsa extract
Hu sheep
rumen fermentation
rumen microorganisms
intestinal microorganisms
