摘要
苞叶数目是衡量玉米品种是否适宜机械直收籽粒的重要性状之一。为研究玉米苞叶数目形态建成的代谢路径及调控基因,本研究以多苞叶数目自交系YD97和少苞叶数目自交系YD132为材料,对7叶期和吐丝期的苞叶组织进行转录组测序。以YD97为对照, YD132为样品,共检测到4089个差异表达基因,其中1979个基因上调表达,2150个基因下调表达。K-means分析将差异表达基因分为8个聚类。GO富集分析表明,差异表达基因主要与刺激响应、氨基酸转运、生长素响应、分枝形态建成、氮化合物代谢过程和细胞周期等相关。KEGG富集分析发现,差异表达基因主要富集于光合作用、植物激素信号转导、淀粉与蔗糖代谢和马达蛋白等途径。其中,植物激素信号转导途径,尤其是生长素信号转导途径,在玉米苞叶数目形态建成中发挥重要作用。此外,将转录组结果与QTL定位结果联合,结合候选基因关联分析和25个玉米自交系苞叶组织的实时荧光定量PCR分析,鉴定到2个玉米苞叶数目候选基因, Zm00001eb156610和Zm00001eb275220,分别编码谷氨酸合成酶2和蛋白质S-酰基转移酶。研究结果有助于玉米苞叶数目的分子机制解析和遗传改良。
Husk number is a key trait influencing the suitability of maize varieties for mechanical grain harvesting.To investigate the molecular mechanisms and candidate genes associated with husk number,the multi-husk inbred line YD97 and the few-husk inbred line YD132 were used as experimental materials.Transcriptome sequencing was conducted on husk tissues collected at the seven-leaf stage and the silking stage.Using YD97 as the control and YD132 as the sample,a total of 4089 differentially expressed genes(DEGs)were identified,including 1979 upregulated and 2150 downregulated genes.K-means clustering grouped these DEGs into eight distinct clusters.Gene ontology(GO)enrichment analysis showed that the DEGs were primarily involved in responses to stimuli,amino acid transport,auxin response,branch morphogenesis,nitrogen compound metabolism,and cell cycle processes.Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis revealed that the DEGs were significantly enriched in pathways related to photosynthesis,plant hormone signal transduction,starch and sucrose metabolism,and motor proteins.Notably,the plant hormone signal transduction pathway—particularly the auxin signaling pathway—appears to play a critical role in the morphogenesis of maize husk number.Furthermore,two candidate genes,Zm00001eb156610(encoding glutamate synthase 2)and Zm00001eb275220(encoding a protein S-acyltransferase),were identified by integrating transcriptome sequencing,QTL mapping,candidate gene association analysis,and quantitative real-time PCR validation across husk tissues from 25 maize inbred lines.These findings contribute to a better understanding of the molecular mechanisms regulating husk number in maize and provide valuable insights for its genetic improvement.
作者
马亮
马璐
张舒钰
章慧敏
王仁明
宋旭东
张振良
冒宇翔
陆虎华
陈国清
郝德荣
周广飞
Ma Liang;Ma Lu;Zhang Shu-Yu;Zhang Hui-Min;Wang Ren-Ming;Song Xu-Dong;Zhang Zhen-Liang;Mao Yu-Xiang;Lu Hu-Hua;Chen Guo-Qing;Hao De-Rong;Zhou Guang-Fei(Jiangsu Yanjiang Institute of Agricultural Sciences,Nantong 226012,Jiangsu,China;Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing 210095,Jiangsu,China;Qingdao Agro-Unitek Import&Export Co.,Ltd.,Qingdao 266100,Shandong,China)
出处
《作物学报》
北大核心
2026年第3期790-801,共12页
Acta Agronomica Sinica
基金
江苏省科协青年科技人才托举工程项目(TJ-2023-052)
江苏省自然科学基金项目(BK20241833)
江苏省重点研发计划项目(BE2022343)
江苏省种业振兴揭榜挂帅项目(JBGS [2021] 054)
江苏沿江地区农业科学研究所青年博士基金项目(YJBS (2023) 004)资助。
关键词
玉米
苞叶数目
转录组测序
植物激素
候选基因
maize
husk number
transcriptome sequencing
plant hormone
candidate gene
