摘要
利用“豫玉 2 2”构建的 2 6 6个玉米F2∶3家系为材料 ,通过一年两点的随机区组田间试验和分子标记分析 ,研究了玉米穗位高、雄穗分支数、茎粗、抽雄期、吐丝期 5个重要农艺性状。相关分析表明 ,穗位高、雄穗分支数、茎粗与单株产量显著正相关 ,抽雄期与吐丝期高度正相关 ,雄穗分支数与茎粗显著正相关。采用复合区间作图法 ,通过5 0 0次排列测验分别确定各性状的LOD阈值 ,在武汉和襄樊两地共定位了 7个穗位高QTL、9个雄穗分支数QTL、8个茎粗QTL、9个抽雄期QTL和 7个吐丝期QTL ;这些QTL在染色体上分布不均匀 ,具有集中分布的特点。研究表明 。
Agronomic traits have significant influence on stability and adaptability in maize production.In this investigation,using a population with 266 F_(2∶3) families from Yuyu22 (Zong3×87-1),two-location field tests were conducted in Wuhan and Xiangfan in 2001,with a randomized complete block design,to characterize five agronomic traits: ear height,tassel branch number,stalk diameter,days to pollen,and days to silk.Correlation analysis of field performance indicated that ear height,tassel branch number and stalk diameter were significantly positive correlative with single-plant yield,days to pollen and days to silk were highly positive correlative with each other,and tassel branch number was significantly positive correlative with stalk diameter too.Utilizing data of field tests and molecular markers,Composite Interval Mapping (CIM) method was used to localize the quantitative trait loci of these traits and 500 times permutation test was conducted to have proper LOD threshold value.As the results,total seven QTL of ear height,nine QTL of tassel branch number,eight QTL of stalk diameter,nine QTL of days to pollen,and seven QTL of days to silk were mapped on 10 chromosomes of maize; all of these QTL distributed unevenly on chromosomes and trended to cluster together.According to analysis of this investigation,the phenotype correlations of quantitative traits may result from the correlations of QTL controlling those traits.Those will be helpful to further understand genetic basis of agronomic traits in maize.
基金
国家重大基础研究发展规划 (编号 :2 0 0 1CB10 880 1)
86 3现代农业主题项目~~
关键词
玉米
农艺性状
QTL
maize
agronomic traits
quantitative trait loci (QTL)
