摘要
目的:为明确黄精多糖调控血糖功效及作用机制,采用网络药理学及分子对接技术对黄精多糖调控血糖的潜在关键成分及作用机制进行探讨。方法:通过TCMSP、PubChem、SwissTargetPrediction数据库获取黄精多糖成分信息和作用靶点,基于Genecard、Drugbank数据库获取疾病作用靶点,利用String数据库分析黄精多糖作用靶点和糖尿病疾病相关靶点的蛋白互作网络(Protein-protein Interaction,PPI)信息。通过Cytoscape3.7.2进行作用靶点间及活性成分-作用靶点网络关系构建。基于京都基因与基因组百科全书(KEGG)进行富集分析。结果:黄精多糖主要由岩藻糖、阿拉伯糖、鼠李糖、半乳糖、葡萄糖、甘露糖、木糖、核糖、果糖、半乳糖醛酸、葡萄糖醛酸组成,单糖成分作用靶点64个,黄精多糖与糖尿病共有靶点57个,PPI网络中半乳糖醛酸、甘露糖、半乳糖、鼠李糖等为核心成分,KEGG通路主要富集于神经活性配体-受体相互作用、5-羟色胺能突触通路、过氧化物酶体增殖物激活受体(PPARs)信号通路等。结论:黄精多糖中半乳糖醛酸、甘露糖、半乳糖等单糖可通过PPARα、HMGCR、半胱天冬酶3(Caspase-3,CASP3)等靶蛋白介导神经活性配体-受体相互作用、5-羟色胺能突触通路、PPARα等通路进行血糖调控。该研究基于网络药理学方法对黄精多糖调控血糖作用机制有了新的认识,为黄精相关功能性产品开发提供理论依据。
【Objective】To elucidate the hypoglycemic effects and mechanisms of Polygonatum sibiricum polysaccharide(PSP),network pharmacology and molecular docking techniques were employed to investigate its potential key components and mechanisms of action in regulating blood glucose.【Method】The component information and action targets of PSP were retrieved from the databases of TCMSP,PubChem and SwissTargetPrediction.Disease-related targets were acquired from Genecard and Drugbank databases.Protein-protein interaction(PPI)information between PSP's action targets and diabetes-related targets was analyzed using the String database.The relationship between action targets and the network of active components-action targets was constructed using Cytoscape 3.7.2.Enrichment analysis was conducted based on the Kyoto Encyclopedia of Genes and Genomes(KEGG).【Result】PSP primarily consists of fucose,arabinose,rhamnose,galactose,glucose,mannose,xylose,ribose,fructose,galacturonic acid and glucuronic acid.There are 64 action targets for the monosaccharide components and 57 shared targets between PSP and diabetes.In the PPI network,galacturonic acid,mannose,galactose and rhamnose emerged as core components.The KEGG pathways were primarily enriched in neuroactive ligand-receptor interaction,serotonergic synapse and peroxisome proliferatoractivated receptors(PPARs)signaling pathway【.Conclusion】galacturonic acid,mannose,and galactose in PSP could regulate glucose levels through HSP90AA1,ACLY,G6PD and other target proteins and thereby mediate neuroactive ligand-receptor interactions,serotonergic synaptic pathways and PPARαpathways.This study provides novel insights into the mechanisms of PSP's hypoglycemic effects using network pharmacology methods,offering a theoretical basis for the development of functional products related to Polygonatum sibiricum.
作者
侯粲
肖杰
王梦倩
贾健斌
安泰
王黎明
郭斐
HOU Can;XIAO Jie;WANG Mengqian;JIA Jianbin;AN Tai;WANG Liming;GUO Fei(COFCO Nutrition and Health Research Institute,Beijing 102209,China;Beijing Key Laboratory of Nutrition&Health and Food Safety,Beijing 102209,China)
出处
《中国食物与营养》
2026年第1期11-17,共7页
Food and Nutrition in China
基金
北京市科技计划项目(项目编号:Z231100004123002)。
关键词
黄精多糖
血糖
网络药理学
分子对接
Polygonatum sibiricum polysaccharides
blood glucose
network pharmacology
molecular docking
