摘要
目的 观察犬尿喹啉酸(KynA)对腹主动脉瘤(AAA)小鼠模型的治疗效果和作用机制。方法 血管紧张素II(Ang II)诱导小鼠发生AAA,将健康雄性ApoE^(–/–)小鼠随机分为假手术组(Sham)、模型组(AAA)和治疗组(AAA+KynA),采用HE染色观察小鼠血管平滑肌细胞(VSMC)的形态变化,TUNEL染色检测VSMC的凋亡情况,免疫荧光检测α-SMA蛋白的表达,Western blot方法检测VSMC中与表型转换和凋亡相关的蛋白表达水平。结果 KynA能降低小鼠的AAA发生率,上调收缩型VSMC标志物α-SMA、SM22α的表达,抑制合成型VSMC标志物骨桥蛋白(OPN)的表达,上调Bcl-2的表达,抑制Bax的表达,抑制VSMC的凋亡。结论 KynA能调节VSMC由合成型到收缩型的转换,并抑制VSMC凋亡。
Objective To observe the therapeutic effect and molecular mechanism of kynurenic acid(KynA)on mice model of abdominal aortic aneurysm(AAA).Methods AngiotensinⅡ(AngⅡ)induces abdominal aortic aneurysm(AAA)in mice.Healthy male ApoE-/-mice were randomly divided into sham group(Sham),model group(AAA),and treatment group(AAA+KynA).HE staining was used to observe the morphological changes of vascular smooth muscle cells(VSMC).TUNEL staining was used to detect the apoptosis of VSMC.The immunofluorescence was used to detect the expression ofα-SMA.Western blot was used to detect the expressions of proteins related to phenotype transition and ap-optosis in VSMC.Results KynA could reduce the incidence of AAA in mice,up-regulate the expressions of contractile VSMC markersα-SMA and SM22α,inhibit the expression of synthetic VSMC marker osteopontin(OPN),up-regulate the expression of Bcl-2,inhibit the expression of Bax,and inhibit the apoptosis of VSMC.Conclusion KynA can regu-late the transformation of VSMC from synthetic to contractile and inhibit VSMC apoptosis.
作者
夏茜
姜宏
于龙
苏峰
宋宾
张立伟
XIA Qian;JIANG Hong;YU Long;SU Feng;SONG Bin;ZHANG Li-wei(Department of Interventional Therapy,General Hospital of Northern Theater Command,People's Liberation Army,Shenyang 110016,China)
出处
《解剖科学进展》
2025年第6期769-771,775,共4页
Progress of Anatomical Sciences
基金
辽宁省科学技术基金(2023-MSLH-362)。
关键词
犬尿喹啉酸
血管平滑肌细胞
腹主动脉瘤
表型转换
凋亡
kynurenic acid
vascular smooth muscle cells
abdominal aortic aneurysm
phenotypic transformation
apoptosis
