摘要
为进一步明确枫香叶抗菌活性物质基础,运用多种色谱技术(如柱色谱、薄层色谱、高效液相色谱等)与波谱分析方法(包括核磁共振波谱、质谱等),对枫香树叶95%乙醇提取物中的化学成分进行分离纯化与结构鉴定,采用杯碟法对其抗菌活性进行评价.从提取物中获得12个化合物,鉴定为槲皮素-3-O-(6″-没食子酰基)-葡萄糖苷(1)、异槲皮苷(2)、槲皮苷(3)、三叶豆苷(4)、山奈酚-3-O-L-呋喃阿拉伯糖苷(5)、山奈酚-3-O-(6″-没食子酰基)-β-葡萄糖苷(6)、紫云英苷(7)、芦丁(8)、杨梅素-3-O-β-D-葡萄糖苷(9)、杨梅素-3-O-(6″-没食子酰基)-吡喃葡萄糖苷(10)、鞣花酸(11)、3,3′-二-O-甲基鞣花酸-4′-O-β-D-木吡喃糖苷(12).化合物5为首次从该植物中分离得到.化合物1、2、6、10和11具有抗菌活性,其中化合物11对革兰氏菌和真菌都有显著的抑制作用,化合物1、2、6和10只对革兰氏菌有抑菌作用.
To further clarify the basis of antimicrobial substances in Liquidambar formosana leaves,various chromatographic techniques(such as column chromatography,thin-layer chromatography,etc.)and spectroscopic methods(including nuclear mag-netic resonance spectroscopy and mass spectrometry,etc.)were employed to separate,purify and identify the chemical constitu-ents in the 95%ethanol extract of l.formosana leaves.The antimicrobial activities were evaluated by Oxford Cup method.Twelve compounds were successfully obtained from the extract,namely quercetin-3-O-(6″-galloyl)-glucoside(1),isoquercitrin(2),quercitrin(3),trifolin(4),kaempferol-3-O-L-arabinofuranoside(5),kaempferol-3-O-(6″-galloyl)-β-glucoside(6),astragalin(7),rutin(8),myricetin-3-O-β-D-glucopyranoside(9),myricetin-3-O-(6″-galloyl)-glucopyranoside(10),ellagic acid(11),and 3,3′-di-O-methyl ellagic acid-4′-O-β-D-xylopyranoside(12).Compound 5 was isolated from this plant for the first time.Antimicrobial activity assay showed that compounds 1,2,6,10,and 11 exhibited antimicrobial activity.Compound 11 showed significant inhibitory effects against both Gram-positive/negative bacteria and fungi.Compounds 1,2,6,and 10 only ex-hibited antimicrobial effects against Gram bacteria.
作者
杨明勋
何潇
崔佳
黄昕玥
李丽梅
YANG Mingxun;HE Xiao;CUI Jia;HUANG Xinyue;LI Limei(School of Pharmacy and Food,Southwest Minzu University,Chengdu 610041,China)
出处
《西南民族大学学报(自然科学版)》
2026年第1期54-59,共6页
Journal of Southwest Minzu University(Natural Science Edition)
基金
国家自然科学基金项目(U23A20516)
中央高校基本科研业务费专项资金项目(ZYN2025238)。
关键词
枫香树叶
黄酮
鞣花酸
抗菌活性
Liquidambar formosana Hance
flavonoids
ellagic acid
antimicrobial
