摘要
目的 研究海洋真菌Talaromyces sp. 13-z-02的次级代谢产物及其抗炎活性。方法 采用硅胶和ODS柱色谱以及半制备液相色谱对发酵产物进行分离和纯化,并利用质谱和核磁共振等方法鉴定化合物的结构。基于斑马鱼模型对分离得到的化合物进行抗炎活性评价。结果 从Talaromyces sp. 13-z-02菌株发酵液中分离鉴定了1个新的色满类化合物以及11个已知化合物,分别为8-羟基-6-甲氧基-7-甲基异色满(1)、芽孢杆菌素B(2)、大孢酮D(3)、芽孢杆菌素F(4)、1-epi-芽孢杆菌素F(5)、大孢酮E与1-epi-大孢酮E(6)、芽孢杆菌素A(7)、异克劳辛(8)、Sch 725680(9)、阿库烯C(10)、6-乙基-2-羟基-4-甲氧基-3-甲基苯甲醛(11)、6-乙基-2,4-二羟基-3-甲苯甲醛基(12)。化合物1和12在20μmol/L时显著降低免疫细胞迁移率(P<0.01)。结论 化合物1为新的色满类化合物,命名为篮异色满;化合物1和12具有抗炎活性。
Objective To investigate the secondary metabolites produced by marine-derived fungi Talaromyces sp.13-z-02 and their anti-inflammatory activities were studied.Methods The isolation and purification of compounds were performed using silica gel and ODS column chromatography,semi-preparative HPLC.And their structures were elucidated by MS and NMR analyses.The isolated compounds were evaluated for their anti-inflammatory activity in zebrafish.Results One new isochromane and eleven known compounds were isolated and identified as 8-hydroxy-6-methoxy-7-methylisochromane(1)from the fermentation broth of this strain,bacillisporin B(2),macrosporusone D(3),bacillisporin F(4),1-epi-bacillisporin F(5),macrosporusone E and 1-epi-macrosporusone E(6),bacillisporin A(7),xenoclauxin(8),Sch 725680(9),aculene C(10),6-ethyl-2-hydroxy-4-methoxy-3-methylbenzaldehyde(11),6-ethyl-2,4-dihydroxy-3-methylbenzaldehyde(12).Compounds 1 and 12 significantly reduced the migration of immune cells with the same concentration of 20μmol/L and exhibited anti-inflammatory activity.Conclusion Compound 1 is a new isochromane,designated as talaroslisochromane.Compounds 1 and 12 showed anti-inflammatory activity.
作者
许睿
李子林
郑圣䶮
王见见
李佳浔
王颖
高文鹏
张洁
黄宗宏
孔凡栋
王聪
李培海
XU Rui;LI Zilin;ZHENG Shengyan;WANG Jianjian;LI Jiaxun;WANG Ying;GAO Wenpeng;ZHANG Jie;HUANG Zonghong;KONG Fandong;WANG Cong;LI Peihai(Key Laboratory of Chemistry and Engineering of Forest Products,State Ethnic Affairs Commission,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Engineering Research Center of Low-carbon and High-quality Utilization of Forest Biomass,University of Guangxi,School of Chemistry and Chemical Engineering,Guangxi Minzu University,Nanning 530006,China;Engineering Research Center of Zebrafish Models for Human Diseases and Drug Screening of Shandong Province,Biology Institute,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250103,China)
出处
《中草药》
2026年第2期405-413,共9页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金青年项目(82204276)
广西自然科学基金项目(2025GXNSFAA069557)
广西高等学校千名中青年骨干教师培育计划
自治区级大学生创新创业训练计划(S202310608216)。
