摘要
壳聚糖(chitosan)是一种天然多糖,可作为杀菌剂和诱抗剂。西瓜小西葫芦黄花叶病毒病(zucchini yellow mosaic virus,ZYMV)是一种严重影响西瓜产量和品质的病害,目前并无有效的防治方法。为了明确壳聚糖对西瓜ZYMV的防控效果,本研究改进了壳聚糖的使用方法,从单一喷施处理改为种子浸泡加幼苗喷施。结果表明,100、200、400、600 mg·L^(-1)壳聚糖均能显著促进西瓜幼苗的生长;在此基础上对西瓜幼苗人工接种ZYMV,4种浓度壳聚糖处理的西瓜幼苗发病率、发病指数均显著低于无菌水对照;其中,600 mg·L^(-1)壳聚糖促生以及防治效果最好;进一步研究抗病机制发现,600 mg·L^(-1)壳聚糖可以显著提高抗氧化酶(PPO、POD、CAT)活性,且西瓜相关抗病基因ClNPR1、ClPR1a、ClWRKY46、ClWRKY30的相对表达量显著提高。综上所述,600 mg·L^(-1)壳聚糖能够增强西瓜抗ZYMV的能力,可以作为一种环境友好的生物源农药应用于农业生产,本研究为西瓜ZYMV防控提供了有力的理论指导。
Chitosan is a natural polysaccharide that can serve as both a fungicide and an elicitor.Zucchini yellow mosaic virus(ZYMV)is a disease that severely affects the yield and quality of watermelon,and currently,there are no effective control methods available.To clarify the preventive and therapeutic effects of chitosan against ZYMV in watermelon,this study improved the application method of chitosan by shifting from a single foliar spray treatment to a combined approach of seed soaking followed by seedling spraying.The results demonstrated that chitosan at concentrations of 100,200,400,and 600 mg·L^(-1)significantly promoted the growth of watermelon seedlings.Subsequently,when these seedlings were artificially inoculated with ZYMV,the incidence rate and disease index of seedlings treated with chitosan at all four concentrations were significantly lower than those of the sterile water control.Among them,chitosan at 600 mg·L^(-1)exhibited the best growth-promoting and disease control effects.Further investigation into the disease resistance mechanism revealed that chitosan at 600 mg·L^(-1)significantly enhanced the activities of antioxidant enzymes(PPO,POD,CAT)and substantially increased the relative expression levels of watermelon disease resistance-related genes(ClNPR1,ClPR1a,ClWRKY46,ClWRKY30).In summary,chitosan at 600 mg·L^(-1)can enhance watermelon’s resistance to ZYMV and can be applied as an environmentally friendly bio-based pesticide in agricultural production.This study provides robust theoretical guidance for the prevention and control of ZYMV in watermelon.
作者
吕文轩
朱冬阳
汤冰倩
卜凤姣
罗玉松
李茂营
戴思慧
LYU Wenxuan;ZHU Dongyang;TANG Bingqian;BU Fengjiao;LUO Yusong;LIMaoying;DAI Sihui(College of Horticulture,Hunan Agricultural University,Changsha 410128,Hunan,China;Vegetable Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Yuelu Mountain Laboratory,Changsha 410082,Hunan,China)
出处
《中国蔬菜》
北大核心
2025年第12期143-151,共9页
China Vegetables
基金
国家西甜瓜产业技术体系项目(CARS-25)。
