摘要
旨在为油茶籽油中多酚类物质的提取提供一种绿色高效的方法,制备了11种天然低共熔溶剂(NADESs),并从中筛选出最适提取油茶籽油多酚的NADESs,在此基础上通过单因素实验和响应面法优化NADESs提取油茶籽油多酚的工艺,并采用液相色谱-串联四极杆质谱联用(LC-MS/MS)技术分析了油茶籽油多酚类物质组成成分。结果表明:以氯化胆碱-丙二醇组成的NADESs的多酚提取效果最佳;最佳提取工艺条件为含水率30%、丙二醇与氯化胆碱物质的量比2.5∶1、超声时间60 min、超声温度30℃,在此条件下多酚含量为38.05 mg/kg;通过LC-MS/MS从多酚提取物中鉴定出20种多酚类物质,其中山柰酚含量最高,为1319.07μg/kg。综上,采用氯化胆碱-丙二醇组成的NADESs可实现油茶籽油中多酚类物质的绿色、高效提取。
Aiming to establish a green and efficient method for extracting polyphenols from camellia seed oil,eleven types of natural deep eutectic solvents(NADESs)were prepared,from which the most suitable NADESs for polyphenol extraction from camellia seed oil was selected.Subsequently,the extraction process was optimized using single-factor experiments and response surface methodology.The polyphenol composition was analyzed by LC-MS/MS.The results showed that the NADESs composed of choline chloride and propylene glycol exhibited the best extraction performance.The optimal extraction conditions were determined as follows:water content 30%,molar ratio of propylene glycol to choline chloride 2.5∶1,ultrasonic time 60 min,and ultrasonic temperature 30℃.Under these conditions,the polyphenol content reached 38.05 mg/kg.A total of 20 polyphenolic compounds in polyphenol extracts were identified by LC-MS/MS,with kaempferol being the most abundant at 1319.07μg/kg.In conclusion,the NADESs composed of choline chloride and propylene glycol enable green and efficient extraction of polyphenols from camellia seed oil.
作者
崔茂凯
孙诗曼
沈丹玉
王文威
周凌原
汤富彬
刘毅华
CUI Maokai;SUN Shiman;SHEN Danyu;WANG Wenwei;ZHOU Lingyuan;TANG Fubin;LIU Yihua(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,China;Nanjing Forestry University,Nanjing 210037,China)
出处
《中国油脂》
北大核心
2026年第2期142-148,共7页
China Oils and Fats
基金
油茶籽油不同形态酚类化合物的精准定量分析及数据库构建(LGC22C160006)
浙江省科学技术厅“领雁”研发攻关计划(2023C02034)。
关键词
天然低共熔溶剂
油茶籽油
多酚
响应面法
natural deep eutectic solvents
camellia seed oil
polyphenols
response surface methodology
