摘要
为分析我国云南边境地区稻种资源的遗传多样性,并发掘与主要农艺性状密切相关的SSR标记,以新收集自我国云南及毗邻的缅甸、老挝的376份稻种资源为材料,分为3个分析单元,观测11个表型性状,采用27对SSR标记进行遗传多样性及与表型性状的关联性分析。结果显示,共检测到142个等位变异位点,其中100个为稀有等位变异位点,9个为特有等位变异位点;基于6个遗传多样性参数的综合评估,3个分析单元稻种资源的得分排序为中国云南分析单元>缅甸分析单元>老挝分析单元;在27对SSR标记中,RM214、RM1086、RM5481、RM6089、RM1509和RM7479与表型性状的关联性较密切,可解释穗长、穗抽出度、穗实粒数、穗秕粒数、穗粒数和千粒重等性状10.0%以上的变化。表型性状的主成分分析提取出4个主成分,累计贡献率为77.218%;筛选出综合得分>1.000的优异资源36份,其中得分>2.000的为花壳、纳列5和毫糯浪,分别具有作为多穗大穗型、大穗型和糯性亲本的潜力。
To analyze the genetic diversity of rice germplasm resources in the border areas of Yunnan Province and identify SSR markers closely related to major agronomic traits,a total of 376 newly collected rice germplasm resources from Yunnan(China),and its adjacent Myanmar and Laos were used as experimental materials.These resources were divided into three analysis units,and 11 phenotypic traits were investigated.Genetic diversity and association with phenotypic traits were analyzed using 27 pairs of SSR markers.Results showed that a total of 142 allelic variation loci were detected,including 100 rare allelic variation loci and nine peculiar allelic variation loci.Based on the comprehensive evaluation of six genetic diversity parameters,the scoring order of rice germplasm resources from the three analysis units was:Yunnan,China analysis unit>Myanmar analysis unit>Laos analysis unit.Among the 27 pairs of SSR markers,RM214,RM1086,RM5481,RM6089,RM1509,and RM7479 were closely associated with phenotypic traits,could explain more than 10.0%of the variation in panicle length,panicle exsertion,filled grains per panicle,unfilled grains per panicle,grains per panicle,and 1000-grain weight.Principal component analysis of phenotypic traits extracted four principal components with a cumulative contribution rate of 77.218%.A total of 36 excellent germplasm resources(comprehensive score≥1.000)were selected.Among these,Huake,Nalei 5,and Haonuolang had a score greater than 2.000.They demonstrated potential as parents for multiple-tillering and large-panicle type,large-panicle type,and glutinous type,respectively.
作者
汤翠凤
阿新祥
董超
张斐斐
杨雅云
杨红梅
戴陆园
苏振喜
Tang Cuifeng;A Xinxiang;Dong Chao;Zhang Feifei;Yang Yayun;Yang Hongmei;Dai Luyuan;Su Zhenxi(Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture and Rural Affairs/Scientific Observation Station for Rice Germplasm Resources of Yunnan,Ministry of Agriculture and Rural Affairs,Kunming 650032,Yunnan,China;Institute of Agricultural Research of Xishuangbanna,Xishuangbanna 666100,Yunnan,China;Institute of Food Crops,Yunnan Academy of Agricultural Sciences,Kunming 650000,Yunnan,China)
出处
《作物杂志》
北大核心
2026年第1期33-46,I0004-I0007,共18页
Crops
基金
国家重点研发计划项目(2021YFD1200505-1)
云南省种子种业联合实验室项目(202205AR070001-11)
云南省重大科技专项计划(202402AE090035)
丽江市苏振喜专家工作站。
关键词
云南边境地区
稻种资源
遗传多样性
SSR标记
关联性分析
Yunnan border areas
Rice germplasm resources
Genetic diversity
SSR marker
Correlation analysis
