摘要
为明确绣球叶斑病的致病菌及其生物学特性,探寻适宜的防治方法,本研究对绣球品种玛梅丽的叶斑病样本进行组织分离,纯化病原菌,基于柯赫氏法则验证,并利用基于ITS、LSU、TEF1等多基因序列构建的系统发育树分析,对病原菌进行鉴定。同时,采用菌丝生长速率法,研究温度、培养基、pH值、碳源、氮源、光周期对病原菌菌丝生长的影响,并测定其致死温度。此外,还测试了7种化学药剂对病原菌的抑制效果。结果显示,分离到的绣球叶斑病病原菌为神农架鹅膏菌(Amanita shennongjiana),其菌丝在25℃、马铃薯葡萄糖琼脂培养基,pH值为5,每日各12 h光、暗交替的条件下生长较好,其最适碳源为蔗糖,偏好不加氮,致死温度为65℃持续10 min。室内环境下,80%乙蒜素和80%波尔多液对该菌的抑制效果较好,其有效中浓度(EC 50)分别为1.9489、1.5918 mg·L^(-1)。本研究是对由神农架鹅膏菌引起的绣球叶斑病在中国的首次报道。
In order to identify the pathogenic fungus of hydrangea leaf spot disease and its biological characteristics,and explore suitable control methods,tissue isolation was conducted on leaf spot samples of hydrangea cultivar Mme E Mouilliere,and the pathogenic fungus was purified,and verified based on Koch's rule.Phylogenetic tree analysis was constructed using multi-gene sequences(ITS,LSU,TEF 1)to identify the pathogenic fungus.Meanwhile,the mycelial growth rate method was adopted to investigate the effects of temperature,culture media,pH value,carbon source,nitrogen source,and photoperiod on the mycelial growth of the pathogenic fungus,and its lethal temperature was determined.Additionally,the inhibitory effects of 7 chemical fungicides on the fungus were tested.The results showed that the pathogenic fungus causing hydrangea leaf spot disease was identified as Amanita shennongjiana.Its mycelia grew well under the following conditions:temperature of 25℃,potato dextrose agar(PDA)medium,pH value of 5,and 12 h light/12 h dark alternation daily.Its optimal carbon source was sucrose,with a preference for nitrogen-free conditions.The lethal temperature of the fungus was 65℃for 10 minutes.Under laboratory conditions,80%ethylicin and 80%Bordeaux mixture exhibited better inhibitory effects on the fungus,with their median effective concentrations(EC 50)being 1.9489 mg·L^(-1)and 1.5918 mg·L^(-1),respectively.This study is the first report of hydrangea leaf spot disease caused by Amanita shennongjiana in China.
作者
陈晶晶
张玉
温联好
刘光炎
周梅
李正敏
熊海燕
杨振翼
杨曾华
童译
王超
CHEN Jingjing;ZHANG Yu;WEN Lianhao;LIU Guangyan;ZHOU Mei;LI Zhengmin;XIONG Haiyan;YANG Zhenyi;YANG Zenghua;TONG Yi;WANG Chao(Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization,Southwest Research Center for Landscape Architecture Engineering(State Forestry and Grassland Administration),Yunnan Province South and Southeast Asia Joint R&D Center of Economic Forest Full Industry Chain,Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province,College of Landscape and Horticulture,Southwest Forestry University,Kunming 650224,China;Kunming Yang Chinese Rose Gardening Co.,Ltd.,Kunming 652501,China)
出处
《浙江农业学报》
北大核心
2026年第1期105-113,共9页
Acta Agriculturae Zhejiangensis
基金
云南省重大科技专项(202202AE090028)
云南省大学生创新训练计划(20200554052)。
