摘要
反硝化依赖型甲烷厌氧氧化(DAMO)是联结碳氮循环的重要过程。本研究开展13C稳定同位素标记微宇宙培养实验,针对长期施肥对红壤水稻土DAMO过程的影响进行了分析。实验设4个处理:不施肥(CK)、氮磷钾肥(NPK)、氮钾肥加猪粪(NKM)和氮磷钾加稻草(NPKS)。结果表明:总DAMO速率对长期施肥有显著响应,其最大值(1762.93 nmol·g^(-1)·d^(-1),以CO_(2)计,下同)和最小值(25.01 nmol·g^(-1)·d^(-1))分别出现在NKM的深层土壤和CK的表层土壤中,并且NKM的表层、中层及深层土壤的DAMO速率均显著高于相应土层的NPKS土壤(P<0.05),分别高出276%、447%和200%。亚硝酸盐型DAMO(n-damo)速率从表层到深层土壤呈递增趋势,NKM的中层土壤n-damo强度显著高于NPKS(高出755%)(P<0.05)。对于硝酸盐型DAMO(Nr-damo)速率,NKM深层土壤(702.49 nmol·g^(-1)·d^(-1))显著高于中层土壤(38.77 nmol·g^(-1)·d^(-1))(P<0.05),而且随土层深度加深,Nr-damo速率在CK中呈现递增、在NPKS中呈现递减的趋势。各处理N_(2)O型DAMO(N_(2)O-damo)速率表现为表层最高(44.23~81.95 nmol·g^(-1)·d^(-1)),深层次之(8.47~18.46 nmol·g^(-1)·d^(-1)),中层最低(0.63~3.27 nmol·g^(-1)·d^(-1))。对于表层土壤N_(2)O-damo速率,NKM显著高于NPKS处理(P<0.05)。研究表明,相比于长期秸秆还田,猪粪配施化肥对红壤水稻土DAMO发生有更强的促进作用;此外,深层水稻土具有较高的ndamo潜力,表层水稻土则具有较高的N_(2)O-damo潜力。
Denitrification-dependent anaerobic methane oxidation(DAMO)is a key process linking carbon and nitrogen cycles.A 13C stable isotope labeling microcosm incubation experiment was conducted to investigate the effects of long-term fertilization on DAMO processes in red paddy soils.Four treatments were established:without fertilizer(CK),chemical fertilizer(NPK:nitrogen,phosphorus,potassium),combined chemical fertilizer and pig manure(NKM),and chemical fertilizer with rice straw incorporation(NPKS).Key findings include:total DAMO rates responded significantly to long-term fertilization.The maximum rate(1762.93 nmol·g^(-1)·d^(-1))and minimum rate(25.01 nmol·g^(-1)·d^(-1))were observed in the deep soil layer of NKM and the surface layer of CK,respectively.Notably,DAMO rates in the surface,middle,and deep soil layers of NKM were 276%,447%,and 200%higher than those in corresponding NPKS layers(P<0.05).Nitrite-dependent DAMO(n-damo)rates increased with soil depth.The n-damo rate in the middle layer of NKM was 755% higher than that in NPKS(P<0.05).Nitrate-dependent DAMO(Nr-damo)rates in the deep soil layer of NKM(702.49 nmol·g^(-1)·d^(-1))were significantly higher than those in the middle layer(38.77 nmol·g^(-1)·d^(-1))(P<0.05).Nr-damo rates increased with depth in CK but decreased in NPKS.4)N_(2)O-dependent DAMO(N_(2)O-damo)rates were highest in surface soils(44.23-81.95 nmol·g^(-1)·d^(-1)),followed by deep layers(8.47-18.46 nmol·g^(-1)·d^(-1)),and lowest in middle layers(0.63-3.27 nmol·g^(-1)·d^(-1)).Surface N_(2)O-damo rates in NKM were significantly higher than those in NPKS(P<0.05).This study demonstrates that pig manure combined with chemical fertilization(NKM)enhances DAMO activity more effectively than straw incorporation(NPKS)in red paddy soils.Additionally,deep soil layers exhibit higher n-damo potential,while surface soils show greater N_(2)O-damo potential.
作者
王娟
吴娟
廖育林
鲁艳红
聂军
万运帆
秦晓波
WANG Juan;WU Juan;LIAO Yulin;LU Yanhong;NIE Jun;WAN Yunfan;QIN Xiaobo(College of Resources and Environment,Qingdao Agricultural University,Qingdao 266109,China;Agricultural Rural Carbon Peak Carbon Neutral Research Center,Chinese Academy of Agricultural Sciences(CAAS),Key Laboratory of Agricultural and Rural Ecological Environment,Ministry of Agriculture and Rural Affairs Institute of Agricultural Environment and Sustainable Development,CAAS,Beijing 100081,China;Soils and Fertilizer Institute of Hunan Province,Changsha 410125,China)
出处
《农业环境科学学报》
北大核心
2026年第1期240-250,共11页
Journal of Agro-Environment Science
基金
中国农业科学院绿色低碳基础科学中心项目(CAAS-CSGLCA-202301)
中国农业科学院重大任务(CAAS-ZDRW202417)
国家重点研发计划项目(2021YFD1700202-05)。
关键词
反硝化型甲烷厌氧氧化
稻田甲烷减排
长期施肥
猪粪
秸秆还田
denitrifying methane anaerobic oxidation
reducing methane emissions in rice paddies
long-term fertilization
pig manure
straw residue return
