摘要
目的研究小米草化学成分及其抗光老化活性。方法采用D101大孔吸附树脂、硅胶、Sephadex LH-20及制备型HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用CCK-8、LDH试剂盒评估抗光老化活性。结果从中分离得到10个化合物,分别鉴定为(5-甲酰基-2-呋喃)甲基二氢阿魏酸酯(1)、十六烷-4-内酯13-O-β-D-吡喃葡萄糖(1→2)-β-D-吡喃葡萄糖苷(2)、芹菜素(3)、7 R,8R-4,7,9,9′-四羟基-3,3′-二甲氧基-8-O-4′-新木脂素(4)、布卢门醇A(5)、阿魏酸(6)、3-苯基乳酸(7)、3-甲氧基-4-羟基苯甲酸(8)、环(脯氨酸-丙氨酸)(9)、5-羟甲基2-呋喃甲醛(10)。化合物1~2可显著提高UVB照射下HaCaT细胞活性,抑制乳酸脱氢酶释放。结论化合物1~2为新化合物,3~10为首次从该植物中分离得到。化合物1~2具有抗光老化活性。
AIM To study the chemical constituents from Euphrasia pectinata Ten.and their anti-photoaging activity.METHODS The separation and purification were carried out by using D101 macroporous adsorption resin,silica gel,Sephadex LH-20 and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-photoaging activity was evaluated by CCK-8 method and LDH kits.RESULTS Ten compounds were isolated and identified as(5-formyl-2-furanyl)methyl dihydrof erulic acid ester(1),16 alkane-4-olide-13-O-β-D-glucopyranosy(1→2)-β-D-glucopyranoside(2),apigenin(3),(7 R,8R-4,7,9,9′-tetrahydroxy-3,3′-dimethoxy-8-O-4′-neolignan)(4),blumenol A(5),ferulic acid(6),p-coumaric acid(7),3-methoxy-4-hydroxybenzo(8),cyclo(Pro-Ala)(9),5-hydroxylmethyl-2-furfural(10).Compounds 1-2 could significantly enhance the viability of HaCaT cells under UVB irradiation,and inhibit the release of lactate dehydrogenase.CONCLUSION Compounds 1-2 are new compounds,3-10 are isolated from this plant for the first time.Compounds 1-2 have anti-photoaging activity.
作者
边子妍
郭浩楠
李花花
郑琦
王涛
韩立峰
李楠
张晗
苗琳
张鹏
BIAN Zi-yan;GUO Hao-nan;LI Hua-hua;ZHENG Qi;WANG Tao;HAN Li-feng;LI Nan;ZHANG Han;MIAO Lin;ZHANG Peng(National Key Laboratory for Chinese Medicine Modernization,Ministry of Education Key Laboratory for Pharmacology of Traditional Chinese Medicine Formulae,Haihe Laboratory for Modern Chinese Medicines,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)
出处
《中成药》
北大核心
2026年第1期114-121,共8页
Chinese Traditional Patent Medicine
基金
国家重点研发计划(2023YFC3504302)。
关键词
小米草
化学成分
分离鉴定
抗光老化活性
Euphrasia pectinata Ten.
chemical constituents
isolation and identification
anti-photoaging activity
