摘要
本文旨在研究桂枝挥发油对肺炎克雷伯菌的体外、体内抑制作用。采用水蒸气蒸馏法提取桂枝挥发油(VORC),琼脂稀释法测定VORC对肺炎克雷伯菌的最低抑菌浓度(MIC);通过生长曲线法检测VORC对肺炎克雷伯菌生长能力的影响;建立肺炎克雷伯菌诱导大鼠重症肺炎模型,探讨VORC在体内的抗菌作用。结果表明,VORC对肺炎克雷伯菌的MIC为2.5 mg/m L,在体外能显著抑制肺炎克雷伯菌的生长,且抑菌活性呈浓度依赖性;临床表现证实了建模成功;灌胃给予高(0.12mL/kg)、中(0.06mL/kg)剂量VORC后,能显著降低大鼠LI值(P<0.01),明显改善动脉血气指标(P<0.01)且能显著降低肺内活菌数(P<0.05)。本研究表明VORC对肺炎克雷伯菌有较好的抑菌效果。
In order to study the antimicrobial effects of volatile oil components of Ramulus Cinnamomi(VORC)on Klebsiella Pneumoniae.VORC was extracted by steam distillation.Minimum inhibition concentrations(MIC)of VORC agansit Klebsiella Pneumoniae were detected by a agar dilution method.The effects of VORC on the growth of Klebsiella Pneumoniae were determined by growth curves.A severe pneumonia mouse model induced by Klebsiella Pneumoniae was established to evaluate the antimicrobial activity of VORC in vivo.Results indicated that the MIC of VORC against Klebsiella Pneumoniae in vitro was 2.5 mg/mL.The growth of Klebsiella Pneumoniae was notably inhibited by different concentration of VORC,with the degree of inhibition positively correlating with drug concentration.The clinical manifestations showed that the rat model of Klebsiella Pneumoniae was set up successfully.Compared with the model group,the level of LI was decreased and the arterial blood gas index could be improved significantly in groups received VORC at the doses of 0.12 mL/kg and 0.06 mL/kg(P<0.01),and the number of viable bacterica in lung was decreased significantly in groups received VORC at the doses of 0.12 mL/kg and 0.06 mL/kg(P<0.05).This study indicates VORC exhibits better bacteriostatic effect on Klebsiella Pneumoniae.
作者
徐军
陈人萍
刘卫
高佳
李会影
张英华
XU Jun;CHEN Renping;LIU Wei;GAO Jia;LI Huiying;ZHANG Yinghua(Jilin Province Academy of Traditional Chinese Medicine,ChangChun 130012,China)
出处
《特产研究》
2026年第1期97-101,111,共6页
Special Wild Economic Animal and Plant Research
基金
吉林省卫生健康科技能力提升计划项目(2024B034)。
关键词
桂枝
挥发油
抑菌作用
肺炎克雷伯菌
Ramulus cinnamomi
volatile oil
antimicrobial effect
Klebsiella pneumoniae
