摘要
为开发生物质基绿色乳化剂,并解决疏水功能分子(如植物多酚、维生素等)在水基体系中分散性差、稳定性低的问题,通过酯化反应将油酸(OA)接枝到半纤维素(H)上制备了两亲性半纤维素-油酸共聚物(H-OA)及其自组装纳米胶束,并将H-OA胶束作软胶体用于稳定油相(大豆油),制备水包油型高内相Pickering乳液(HIPPE),探究其在不同环境应力(长期储存、加热及离心)下的稳定性。以姜黄素(Cur)为模型分子,研究HIPPE在高浓度水平分散疏水性功能分子的潜力。研究结果表明:HIPPE在显微镜下呈现出球形液滴紧密堆叠的结构;由质量浓度15 g/L H-OA稳定的HIPPE,经过常温储存30 d、85℃加热30 min后的液滴粒径约为20μm,乳化指数高达99%,脱油率低于1%;以质量浓度12 g/L H-OA稳定的HIPPE对姜黄素的包封率达到97.4%,所包载的功能分子呈现高活性,DPPH自由基清除率达到92.3%。体外模拟消化实验发现,载药HIPPE(15 g/L H-OA)的生物可及度和游离脂肪酸释放量均优于大豆油,与直接载药的大豆油相比分别增加了35%和40%。
This study aimed to develop a biomass-derived green emulsifier for enhancing the aqueous dispersion and environmental stability of hydrophobic bioactive compounds(e.g.,plant polyphenols,vitamins).By grafting oleic acid(OA)onto hemicellulose(H)via esterification,an amphiphilic copolymer H-OA and its self-assembled micelles were prepared.H-OA micelles were used to stabilize soybean oil to fabricate oil-in-water high internal phase Pickering emulsions(HIPPE).The stability of HIPPE was evaluated under environmental stresses including long-term storage,high temperature and centrifugation.Taking curcumin(Cur)as the model molecule,the potential of HIPPE for high-concentration dispersion of hydrophobic functional molecules was investigated.HIPPE exhibited tightly packed spherical droplets under microscopy.The droplet sizes of HIPPE stabilized with 15 g/L H-OA were about 20μm after 30 d storage and heating at 85℃for 30 min,showing an emulsification index exceeding 99%and oil removal rate below 1%.HIPPE stabilized with 12 g/L H-OA achieved 97.4%curcumin encapsulation efficiency,with encapsulated molecules retaining high bioactivity(DPPH radical scavenging rate of 92.3%).In vitro digestion simulations revealed that compared to drug-loaded soybean oil,the bioavailability and free fatty acid release of drug-loaded HIPPE(15 g/L H-OA)increased by 35%and 40%,respectively.
作者
林政明
杜桂涛
余晗
沈锋
王小慧
LIN Zhengming;DU Guitao;YU Han;SHEN Feng;WANG Xiaohui(School of Light Industry Science and Engineering,South China University of Technology,State Key Laboratory of Advanced Papermaking and Paper-based Materials,Guangzhou 510640,China;Jinan Shengquan Group Co.,Ltd.,Jinan 250200,China)
出处
《林产化学与工业》
北大核心
2026年第1期45-55,共11页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(32171721)
山东省自然科学基金资助项目(ZR2023QB282)
广东省基础与应用基础研究基金委员会卓越青年团队项目(2023B1515040013)。
