摘要
目的:建立同时测定尿感宁颗粒中新绿原酸、秦皮甲素、绿原酸、隐绿原酸、秦皮乙素、咖啡酸、木犀草素-7-O-二葡萄糖醛酸苷、芹菜素-7-O-二葡萄糖醛酸苷、忍冬苷、野漆树苷、迷迭香酸11个成分含量的高效液相色谱(HPLC)方法。方法:采用Waters XTerra®MS C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长330 nm,柱温30℃。结果:11个成分在各自相应浓度范围内与峰面积呈现良好的线性关系,r均>0.9996;精密度、重复性、稳定性(RSD均<2.0%)良好;平均回收率为96.6%~101.7%,RSD为0.6%~1.9%;8批样品中新绿原酸、秦皮甲素、绿原酸、隐绿原酸、秦皮乙素、咖啡酸、木犀草素-7-O-二葡萄糖醛酸苷、芹菜素-7-O-二葡萄糖醛酸苷、忍冬苷、野漆树苷和迷迭香酸含量范围分别为0.29~1.21、0.19~0.48、0.30~1.16、0.28~1.16、5.75~11.05、0.37~2.35、0.84~3.14、0.46~3.47、1.58~4.37、0.44~1.57和1.36~6.13 mg·袋^(-1)。结论:该研究建立的方法简便易行,重复性好,可用于尿感宁颗粒的质量控制。
Objective:To establish a high performance liquid chromatography(HPLC)method for the simultaneous determination of eleven chemical components including neochlorogenic acid,esculin,chlorogenic acid,cryptochlorogenic acid,aesculetin,caffeic acid,luteolin-7-O-diglucuronoside,apigenin-7-O-diglucuronoside,lonicerin,rhoifolin,and rosmarinic acid in Niaoganning granules.Methods:The Waters XTerra^(R)C_(18) column(250 mm×4.6 mm,5μm)was used for gradient elution with the mobile phase composed of acetonitrile(A)and 0.1%phosphoric acid(B)at the flow rate of 1.0 mL·min^(-1).The detection wavelength was set as 330 nm,and the column temperature was at 30 C.Results:The eleven chemical components showed good linear relationships between concentrations within the corresponding ranges and peak areas,with the r values all above 0.9996.The established method demonstrated high precision,repeatability,and stability,with all relative standard deviations(RSDs)<2.0%.The average recoveries were 96.6%-101.7%,with RSDs of 0.6%-1.9%.The contents of neochlorogenic acid,aesculin,chlorogenic acid,cryptochlorogenic acid,aesculetin,caffeic acid,luteolin-7-O-diglucuronoside,apigenin-7-Odiglucuronoside,lonicerin,rhoifolin,and rosmarinic acid in eight batches of samples were 0.29-1.21 mg per bag,0.19-0.48 mg per bag,0.30-1.16 mg per bag,0.28-1.16 mg per bag,5.75-11.05 mg per bag,0.37-2.35 mg per bag,0.84–3.14 mg per bag,0.46-3.47 mg per bag,1.58-4.37 mg per bag,0.44-1.57 mg per bag,and 1.36-6.13 mg per bag,respectively.Conclusion:This established method is simple and reproducible and can thus be used for the quality control of Niaoganning granules.
作者
俞燕
熊昆
王毓
余沙
袁铭铭
雷铭道
周雷罡
肖林林
YU Yan;XIONG Kun;WANG Yu;YU Sha;YUAN Ming-ming;LEI Ming-dao;ZHOU Lei-gang;XIAO Lin-lin(Jiangxi Maternal and Child Health Hospital,Nanchang 360006,China;NMPA Key Laboratory of Quality Evaluation of Chinese Patent Medicine,Jiangxi Province Engineering Research Center of Drug and Medical Device Quality,Jiangxi Institute for Drug Control,Nanchang 330029,China;Pingshan District Maternal and Child Health Hospital of Shenzhen,Shenzhen 518118,China;The People's Hospital of Ganzhou,Ganzhou 341000,China)
出处
《药物分析杂志》
2025年第12期2052-2059,共8页
Chinese Journal of Pharmaceutical Analysis
基金
江西省中医药管理局科技计划项目(2024B1372)
江西省医院药学专项研究基金资助项目(2024-ZXYJ07)。
关键词
尿感宁颗粒
高效液相色谱
连钱草
凤尾草
紫花地丁
秦皮乙素
忍冬苷
迷迭香酸
含量测定
Niaoganning granules
high performance liquid chromatography
Glechomae Herba
Pteris Multifidae Herba
Violae Herba
aesculetin
lonicerin
rosmarinic acid
content determination
