摘要
黄花菜(Hemerocallis citrina)因其营养价值与药用潜力被广泛食用,但其是否含有秋水仙碱(Colchicine)类毒性结构仍存在学术争议。为探究该问题,本研究采用傅里叶变换红外光谱(FTIR)技术,对鲜黄花菜不同部位(根、茎、花)中秋水仙碱类结构的存在性及相对表达水平进行分析。通过引入秋水仙碱标准品和阳性对照植物秋水仙球茎样品,构建酰胺C=O、甲氧基C—O、醚键C—O—C等典型功能团的特征峰识别体系,实现结构的快速筛查。在此基础上,提出基于六个关键特征峰的吸光度与面积归一比值分析方法,并构建特征峰组合指数(Colchicine-related Expression Index,CEI)模型,用于评估目标结构的相对表达强度。结果表明,花部样品在各功能区的特征峰响应最强,CEI值显著高于茎和根部,提示花部可能为秋水仙碱类结构的主要潜在富集区域。本研究提出的“结构识别-比值计算-组合建模”分析策略,为复杂植物样品中微量毒性结构的快速识别与表达强度估算提供了光谱学支持,具备良好的应用推广前景。
Hemerocallis citrina is widely consumed for its nutritional and medicinal benefits.However,whether it contains colchicine or colchicine-like toxic structures remains a subject of scientific debate.In this study,Fourier transform infrared spectroscopy(FTIR)was employed to investigate the presence and relative expression levels of colchicine-related structures in different parts of H.citrina(root,stem,and flower).A spectral reference system was established using a colchicine standard and Colchicum autumnale corm(a known colchicine-rich plant)to identify functional group-specific absorption peaks,including amide C=O,methoxy C—O,and ether C—O—C.Six characteristic peaks were selected to perform normalized absorbance and peak area ratio analyses.Furthermore,a composite metric—Colchicine-related Expression Index(CEI)—was developed to semi-quantitatively estimate the structural expression intensity across tissues.The results showed that the flower exhibited the strongest absorption features and the highest CEI values,suggesting it as a potential accumulation site of colchicine-like structures.This work introduces a spectral strategy integrating functional group recognition,multi-peak ratio modeling,and index-based evaluation,offering a rapid and non-destructive tool for trace-level toxic structure screening in complex plant matrices.
作者
刘艳
王冬冬
邹亚峰
司民真
LIU Yan;WANG Dongdong;ZOU Yafeng;SI Minzhen(Institute of Spectroscopic Application Technology,Chuxiong Normal University,Key Laboratory of Molecular Spectroscopy in Yunnan Provincial Universities,Chuxiong,Yunnan 675000,China;Chuxiong No.1 High School,Chuxiong,Yunnan,675000,China)
出处
《光散射学报》
北大核心
2025年第4期725-735,共11页
The Journal of Light Scattering
基金
国家自然科学基金(13064001,10864001)
2021年云南省大学生创新创业训练计划建设项目(202111391031)。
