摘要
为解析蝴蝶兰属原生种质的遗传多样性并构建其分子身份证,本研究选用43份蝴蝶兰原生种质为试验材料,采用TP-M13-SSR分子标记技术对其遗传多样性进行分析,并基于SSR指纹图谱为每份种质构建分子身份证。结果表明:1)基于简化基因组测序数据开发的SSR引物中筛选出的26对引物适用于本试验材料的扩增,其中24对扩增结果呈高多态性;26对引物的平均观测等位基因数(Na)为2.681,平均有效等位基因数(Ne)为1.894,平均期望杂合度(He)为0.323,平均观测杂合度(Ho)为0.279,所有位点均为He>Ho,Shannon指数(I)均值为0.581,说明43份蝴蝶兰原生种质的杂合程度极低,遗传多样性不高,这可能与所用实验材料均为原生种或其变型和变种相关。2)本试验筛选出的引物适用于对蝴蝶兰属原生种质进行分类鉴评,43份种质的聚类结果和PCoA分析结果均与目前最新的植物学分类结果基本一致。3)根据扩增结果筛选出6对核心引物,可构建每份种质特有的分子身份证。综上,本研究实现了蝴蝶兰原生种质资源的快速分子鉴定,为后续蝴蝶兰属种质资源的收集保存、鉴评和开发利用奠定了基础。
To analyze the genetic diversity of original Phalaenopsis germplasms and construct molecular identities,43 samples were selected as test materials.TP-M13-SSR technology was employed to analyze their genetic diversity and construct molecular identities based on SSR fingerprinting.The results showed that:1)Among the 26 pairs of SSR polymorphic universal primers screened from those developed SSR primers based on simplified genome sequencing data,24 pairs showed high polymorphism in the amplification of the experimental materials.The average number of observed alleles(Na)for the 26 primers was 2.681,the average number of effective alleles(Ne)was 1.894,the average expected heterozygosity(He)was 0.323,and the average observed heterozygosity(Ho)was 0.279.All loci showed He>Ho,and the average Shannon index(I)was 0.581.The results indicate that the level of heterozygosity in the test materials is extremely low,and genetic diversity is not high,which may be related to the fact that all experimental materials were original species,their forms,or varieties.2)The primers screened in this experiment are suitable for the classification and evaluation of original Phalaenopsis germplasms.The clustering results and PCoA analysis of the 43 germplasm samples were consistent with the latest botanical classification of Phalaenopsis germplasms.3)Based on the amplification results,six pairs of core primers were selected to construct unique molecular ID for each germplasm.This study achieved rapid molecular identification of Phalaenopsis germplasm resources,laying a foundation for the subsequent collection,preservation,evaluation,and utilization of Phalaenopsis germplasm resources.
作者
肖文芳
陈和明
吕复兵
XIAO Wenfang;CHEN Heming;LYU Fubing(Environmental Horticulture Research Institute/Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)
出处
《中国农业大学学报》
北大核心
2026年第2期104-115,共12页
Journal of China Agricultural University
基金
广东省科技计划项目(2024B1212060012)
国家重点研发计划(2023YFD230090402)
广州市科技计划项目(2024E04J1267)。
