摘要
为更加科学、快捷、精准地开展梨品种资源鉴定工作,文章综述了SSR指纹图谱身份证技术,回顾了该项技术在梨品种鉴定方面的研究进展和应用情况,详细分析了当前这项技术在简便性、可扩充性、标记数据的采集与分析等方面存在的问题,针对上述问题介绍了采用更高效的编码方法、进行数据扩充、使用荧光标记毛细管电泳检测技术等改进的方向,并对今后SSR指纹图谱身份证技术结合新一代测序技术,实现更加快速、准确、高效的梨品种鉴定工作等方面进行了展望。
In order to carry out pear variety resource identification work more scientifically,quickly and accurately,the article reviewed the SSR fingerprinting ID technology,the research progress and application of this technology in pear variety identification,and analyzed in detail the current problems of this technology in terms of simplicity,expandability,and collection and analysis of tagged data,etc.To address the above problems,the article introduced the direction of improvement such as using more efficient coding methods,data expansion,and fluorescent labelling capillary electrophoresis detection technology,etc.The article also looked forward to the future of SSR fingerprint ID technology combined with the new generation of sequencing technology,to achieve faster,more accurate and more efficient identification of pear varieties.
作者
曹军
王茂森
王国铸
赵杰
王建明
郑思思
陶弢
赵怡宁
张才喜
纠松涛
CAO Jun;WANG Maosen;WANG Guozhu;ZHAO Jie;WANG Jianming;ZHENG Sisi;TAO Tao;ZHAO Yining;ZHANG Caixi;JIU Songtao(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240;Shanghai Pudong New Area Agricultural Technology Promotion Center,Shanghai 201201)
出处
《中国农学通报》
2025年第4期25-30,共6页
Chinese Agricultural Science Bulletin
基金
上海市科技兴农重点攻关项目“早熟优质梨新品种选育及其分子身份证的创建”(2022-02-08-00-12-F01120)
浦东新区农业技术推广中心财政专项项目“早熟高糖型蜜梨新品种引进及配套栽培技术研究”(NJZX23-08-02)。
关键词
梨
SSR标记
指纹图谱
分子身份证
研究进展
pear
SSR marking
fingerprint spectrum
molecular ID card
research progress
