摘要
目的:探讨外周血结核感染T细胞斑点试验(T-SPOT)、结核抗体(TB-Ab)联合呼吸道标本结核分歧杆菌核酸(TB-DNA)对艾滋病合并肺结核的诊断价值。方法:选取2021年7月-2024年7月萍乡市第二人民医院收治的40例艾滋病合并肺结核患者为观察1组,同期接收的40例单纯肺结核患者为观察2组,同时选取40例健康体检者为对照组。分别采集血液标本和呼吸道标本,检测T-SPOT,TB-Ab和TB-DNA,采用受试者工作特征(ROC)曲线分析T-SPOT,TB-Ab和TB-DNA单项检测及联合检测对艾滋病合并肺结核的诊断价值。结果:观察1组与观察2组患者的T-SPOT,TB-Ab和TB-DNA阳性检出率均高于对照组,差异有统计学意义(P<0.05)。观察2组患者的T-SPOT,TB-Ab和TB-DNA阳性检出率均略高于观察1组,但差异无统计学意义(P>0.05)。联合检测对艾滋病合并肺结核患者的阳性检出率均高于3项指标单项检测,差异有统计学意义(P<0.05)。联合检测对健康体检者的阳性检出率低于TB-Ab单项检测,差异有统计学意义(P<0.05)。联合检测诊断艾滋病合并肺结核的曲线下面积(AUC)均高于3项指标单项检测(P<0.05)。结论:T-SOPT,TB-Ab和TB-DNA对艾滋病合并肺结核有良好的诊断作用,联合检测可进一步提高阳性检出率及诊断效能。
Objective:To explore the diagnostic value of peripheral blood tuberculosis infection T-cell spot test(T-SPOT),tuberculosis antibody(TB-Ab)combined with nucleic acid of Mycobacterium tuberculosis(TB-DNA)in respiratory tract specimens for AIDS complicated with pulmonary tuberculosis.Methods:Forty patients with AIDS complicated with pulmonary tuberculosis admitted to the Second People's Hospital of Pingxiang City from July 2021 to July 2024 were selected as observation Group 1.Forty patients with simple pulmonary tuberculosis admitted during the same period were selected as observation Group 2.Meanwhile,40 healthy individuals undergoing physical examinations were selected as the control group.Blood specimens and respiratory tract specimens of the patients were collected respectively to detect the expressions of T-SPOT,TB-Ab and TB-DNA.The diagnostic value of T-SPOT,TB-Ab,TB-DNA single detection and combined detection in AIDS patients with pulmonary tuberculosis was analyzed by receiver operating characteristic(ROC)curve.Results:The positive detection rates of T-SPOT,TB-Ab and TB-DNA in observation group 1 and observation group 2 were both higher than those in the control group,and the difference was statistically significant(P<0.05).The positive detection rates of T-SPOT,TB-Ab and TB-DNA in both groups of patients were slightly higher than those in group 1,but there was no statistically significant difference between the groups(P>0.05).The positive detection rate of combined detection for AIDS patients with pulmonary tuberculosis was higher than that of single detection of three indicators,with a statistically significant difference(P<0.05).The positive detection rate of joint testing for healthy examinees is lower than that of TB Ab single test,and the difference is statistically significant(P<0.05).The area under the curve(AUC)of AIDS patients with pulmonary tuberculosis diagnosed by combined detection was higher than that of single detection of three indicators(P<0.05).Conclusion:T-SOPT,TB-Ab and TB-DNA have good diagnostic effects in AIDS complicated with pulmonary tuberculosis.The combined detection can further improve the positive detection rate and diagnostic efficacy,and is suitable for clinical promotion and application.
作者
钟希
漆阳红
李根
ZHONG Xi;QI Yanghong;LI Gen(Second People's Hospital of Pingxiang,Pingxiang 337000,China)
出处
《临床医药实践》
2026年第2期123-127,共5页
Proceeding of Clinical Medicine
基金
萍乡市科技计划项目(课题编号:2024PY093)。
关键词
艾滋病
肺结核
结核感染T细胞斑点试验
结核抗体
结核分歧杆菌核酸
acquired immunodeficiency syndrome
tuberculosis
tuberculosis infection T-cell spot test
tuberculosis antibodies
nucleic acid of mycobacterium tuberculosis divergences
