摘要
目的:观察赶黄草复方对酒精性脂肪肝大鼠血清学指标、丙二醛(MDA)、黄嘌呤氧化酶(XO)和血小板衍生生长因子(PDGF-bb)活力的影响。方法:选取75只SD大鼠,随机分配至3个组别,分别是对照组(25只)、西药治疗组(30只)和中药治疗组(20只)。各组均以高脂饲料+酒精灌胃造模(浓度梯度按40%/4周、45%/4周、50%/4周递增),于第9周起中药组大鼠酒精灌胃半小时后予中药浸膏2 mL(1.33 g/mL),西药组大鼠予易善复混悬液2 mL(多烯磷酸酯胆碱浓度4.56 mg/mL),对照组大鼠接受等量的2 mL生理盐水灌胃处理,持续至第12周末。实验结束时,对所有组别的大鼠进行解剖取样,检测其血清中的AST、ALT、TG和TC水平,酶联免疫吸附测定(ELISA)肝匀浆中丙二醛(MDA)、黄嘌呤氧化酶(XO)的含量,ELISA法测定PDGF-bb活力,HE染色后行病理学观察。结果:与对照组相比,中药组在ALT、AST、TG和TC这4个指标上均显示出了降低趋势(P<0.01)。而在西药组中,仅TG水平与对照组有显著差异(P<0.05)。当对比中药组和西药组时,中药组在ALT水平上的改善更为显著(P<0.01),同时在AST和TG水平上也有显著差异(P<0.05),但在TC水平上两组间无显著性差异。尽管中药组、西药组和对照组在MDA、XO和PDGF-bb这3个指标上的比较结果均未达到统计学显著性(P>0.05)。然而,中药组在这3个指标上的值均为3组中最低。结论:赶黄草复方可改善大鼠肝生化指标,对实验性大鼠酒精性脂肪肝有一定的保护作用,且其疗效要优于西药易善复,其作用机制可能与降低MDA、XO和PDGF-bb含量有关。
Objective We observed the influence of GHCFF on hematological parameters,malonaldehyde(MDA),xanthine oxi-dase(XO),and platelet-derived growth factor(PDGF).Methods We conducted the study from April 2011 to May 2012.The 75 SD rats were randomly divided into 3 groups:the control group(25 rats),the essential N group(30 rats),and the GHCFF group(20 rats).Rats had free access to a high-release rodent diet and tap water.From the first week to the 12th week,the rats were given 40%,45%,and 50%gradient-concentration alcohol by intragastric administration.From the 9th week,after half an hour of the first alcoholic intragastric administration,the GHCFF group was given the traditional Chinese medicine extract 2 mL(GHCFF,1.33 g/mL),the Essentiale N group was given Essentiale N supply 2 mL(polyene phosphate ester bilirubin,4.56 g/mL),the control group was given isotonic Na chloride 2mL intragastric administration,to the last of 12th week.At the end of the study,all rats from each group were sacrificed,and serum levels of AST,ALT,TG,and TC were measured,the PDGF-bb vigor and the contents of MDA and XO were detected by enzyme-linked immunosorbent assay(ELISA).HE-dyeing,and then the liver was observed pathological change under a microscope.Result Compared with the control group,the four indexes of ALT,AST,TG and TC in TCM group showed a decreasing trend(P<0.01).In the Essentiale N group,only TG levels showed a significant difference from the control group(P<0.05).When comparing the GHCFF group with the Essentiale N group,the GHCFF group had a more significant improvement in ALT levels(P<0.01).Also,it showed significant differences in AST and TG levels(P<0.05),but there was no significant difference in TC levels between the two groups.Although the comparisons of MDA,XO,and PDGF-bb levels between the GHCFF group,Essen-tiale N group,and control groups did not reach statistical significance(P>0.05),the values of these three indicators in the GHCFF group were the lowest among the three groups.Conclusion GHCFF Compound can affect liver biochemical markers and protect experi-mental alcoholic fatty liver in rats.Its efficacy is superior to that of the Essentiale N,and its mechanism is related to reducing MDA,XO,and PDGF-bb levels.
作者
李凤丽
王进博
刘文倩
韩锦卉
柴士伟
LI Fengli;WANG Jinbo;LIU Wenqian;HAN Jinhui;CHAI Shiwei(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;National Clinical Research Center for Chinese Medicine,Tianjin 300193,China;National Committee on the Assessment of the Protected Traditional Chinese Medicinal Products,Beijing 100070,China;China Pharmaceutical University,Nanjing 210009,China)
出处
《中国民族民间医药》
2026年第1期38-45,共8页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
河北省中医药管理局科研计划项目(T2026088)。
