摘要
为建立快速、灵敏的检测赭曲霉毒素A(ochratoxin A,OTA)的方法,通过冷冻法将胶体金与互补链进行偶联作为金标探针(AuNPs-SH-cDNA)固定于结合垫上,抗地高辛单克隆抗体和适配体分别作为T线和C线固定于硝酸纤维素膜上,地高辛修饰的核酸适配体作为识别元件用于结合上样缓冲液中的OTA。在最优条件下,该方法的检出限值为0.3 ng/mL,半数抑制浓度为12.5 ng/mL,该试纸条对玉米样品的加标回收率在100.82%~106.30%之间,变异系数在2.54%~6.78%之间,假阳性率≤0%、假阴性率≤1%。本研究制备的层析试纸条能够成为玉米中OTA检测的有效方法,具有良好的应用前景。
To establish a rapid and sensitive method for detecting ochratoxin A(OTA),a lateral flow test strip was developed using a gold nanoparticle-cDNA conjugate(AuNPs-SH-cDNA)by a freezing method as the detector probe on the conjugate pad as well as using anti-digoxin monoclonal antibodies as the test line and aptamers as the control line on the nitrocellulose membrane.Digoxin-modified aptamers(Dig-Apt)were used as recognition elements for binding to OTA in the loading buffer.Under optimal conditions,the limit of detection(LOD)of the method was 0.3 ng/mL,and the half-maximal inhibitory concentration(IC50)was 12.5 ng/mL.The recoveries in spiked corn samples ranged from 100.82%to 106.30%,with coefficients of variation between 2.54%and 6.78%,a false positive rate≤0%and a false negative rate≤1%.The developed test strip provides an effective method for detecting OTA in corn,demonstrating promising application potential.
作者
李行言
任文洁
王玉龙
王志华
韩雨秀
李佳妮
任凯婵
何保山
LI Xingyan;REN Wenjie;WANG Yulong;WANG Zhihua;HAN Yuxiu;LI Jiani;REN Kaichan;HE Baoshan(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China;Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《食品科学》
北大核心
2026年第1期8-16,共9页
Food Science
基金
河南工业大学“双一流”本科生科技创新能力提升专项(HN-HautFood IAEG-015)
国家自然科学基金青年科学基金项目(32402234)
河南省科技开发联合基金项目(225200810065)
河南省教育厅项目(25B550002)。
