摘要
阳离子/质子反转运蛋白CPA(Cation Proton Antiporter)超家族参与调节植物细胞内离子稳态和pH,在植物抗逆过程中发挥重要作用。禾本科小麦族盐生植物海大麦(Hordeum marinum)具有优异的耐盐和耐渍性。为了系统鉴定海大麦CPA家族成员并解析其结构特征与在盐、渍水胁迫下的表达模式,为阐明其抗逆功能提供理论基础,本研究采用生物信息学方法,在全基因组水平上鉴定海大麦CPA家族成员,并结合系统发育分析、基因结构和保守基序预测、染色体定位、顺式作用元件解析及共线性分析,对其进化与功能特征进行系统研究。同时基于盐胁迫与渍水胁迫的RNA-seq数据,评估海大麦CPA家族基因的表达响应模式。结果显示:从海大麦基因组中共鉴定到32个海大麦CPA成员,可分为NHX、KEA和CHX三大亚家族,三者在基因结构与基序数量上存在显著差异。染色体定位与共线性分析表明,该基因家族主要通过全基因组复制事件扩张。转录组分析显示,在盐胁迫下有超过一半的海大麦CPA家族基因显著差异表达,而在渍水胁迫条件下无基因显著差异表达。本研究构建了海大麦CPA家族的系统框架,为进一步阐明海大麦CPA家族基因在非生物胁迫响应中的分子机制提供了理论依据和参考。
The cation/proton antiporter(CPA)superfamily plays vital roles in maintaining intracellular ion homeostasis and pH balance,thereby contributing to plant tolerance to various abiotic stresses.Hordeum marinum,a halophytic species within the Triticeae tribe,exhibits remarkable salt and waterlogging tolerance.In this study,we systematically identified members of the CPA gene family in H.marinum and analyzed their structural features and expression patterns under salt and waterlogging stresses,with the aim of providing a theoretical foundation for elucidating their stress-responsive functions.Using genome-wide bioinformatics approaches,we identified CPA family members and performed comprehensive analyses including phylogenetic relationships,gene structure and conserved motif composition,chromosomal distribution,cisacting regulatory elements,and synteny to reveal their evolutionary and functional characteristics.Furthermore,RNA-seq datasets under salt and waterlogging treatments were employed to evaluate the expression responses of H.marinum CPA family genes.A total of 32 H.marinum CPA members were identified in the H.marinum genome and classified into three subfamilies:NHX,KEA,and CHX,which showed marked differences in gene structure and motif number.Chromosomal mapping and synteny analyses indicated that whole-genome duplication events primarily contributed to the expansion of the CPA family.Transcriptome analysis revealed that more than half of the HmCPA genes were significantly differentially expressed under salt stress,whereas none were significantly regulated under waterlogging stress.This study establishes a systematic framework for the CPA gene family in H.marinum and provides important insights for elucidating their molecular mechanisms in response to abiotic stresses.
作者
张彤彤
吴德志
邝刘辉
ZHANG Tongtong;WU Dezhi;KUANG Liuhui(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China)
出处
《作物研究》
2025年第6期569-580,共12页
Crop Research
基金
湖南省自然科学基金(2024JJ6243)
湖南省教育厅科学研究项目(24B0205)。
