摘要
高尿酸血症(hyperuricemia,HUA)是一种嘌呤代谢紊乱综合症,患病人数逐年上升,目前治疗HUA的药物别嘌呤醇存在肾毒性风险,严重时可危及生命,因此寻找一种安全的降尿酸方法十分必要。该研究通过平板初筛结合HPLC筛选到降解尿酸的菌株凝结魏茨曼氏菌(Weizmannia coagulans)B8,其尿酸降解率为29.12%;W.coagulans B8在体外表现出良好的耐酸性(pH 2.0)和胆盐(0.3%胆盐)耐受性,同时对致病细菌具有广谱的拮抗作用,这些特性为其作为潜在益生菌提供了依据;从基因水平对菌株W.coagulans B8的尿酸代谢机理进行探究,通过全基因组测序分析发现,W.coagulans B8基因组全长3669133 bp,注释到尿酸代谢相关基因(allB、rpoC、purK等)62个,整合到KEGG嘌呤代谢通路,同时测定尿酸酶活力为(1.34±0.16)U/mL,从而阐明尿酸完整代谢途径。研究结果初步揭示了W.coagulans B8的益生特性及尿酸代谢相关基因和途径,为菌株进一步应用于食品中控制体内尿酸水平提供理论基础。
Hyperuricemia(HUA),as a syndrome of purine metabolism disorder,has been increasing in number year by year.At present,allopurinol,a drug used to treat HUA,is nephrotoxic and can even cause death.It is necessary to find a safe method to reduce uric acid.In this study,the strain Weizmannia coagulans B8 with uric acid degradation rate of 29.12%was identified through plate screening combined with HPLC.The strain exhibits good acid resistance(pH 2.0)and bile salt tolerance(0.3%bile salt)in vitro,meanwhile has a broad-spectrum antagonistic effect on pathogenic bacteria.These characteristics provide a basis for its potential as a probiotic.The mechanism of uric acid metabolism in strain W.coagulans B8 was explored at the genetic level.Whole genome sequencing analysis revealed that the genome of W.coagulans B8 is 3669133 bp long,with 62 genes related to uric acid metabolism(allB,rpoC,purK,etc.)annotated and integrated into the KEGG purine metabolism pathway.The uricase activity was also measured to be(1.34±0.16)U/mL,thus elucidating the complete metabolic pathway of uric acid.The results have revealed the probiotic characteristics and uric acid metabolism related genes and pathways of W.coagulans B8,providing a theoretical basis for further application of the strain in controlling uric acid levels in food.
作者
杨蕊
于洋
宋呈英
满都拉
孙子羽
陈忠军
YANG Rui;YU Yang;SONG Chengying;Mandlaa;SUN Ziyu;CHEN Zhongjun(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010000,China)
出处
《食品与发酵工业》
北大核心
2026年第1期113-121,共9页
Food and Fermentation Industries
