摘要
目的探讨白细胞介素(IL)-4/髓系细胞触发受体-2(TREM-2)信号通路相关因子在小鼠外耳道胆脂瘤(EACC)组织的表达及意义。方法15只C57BL6/J正常小鼠随机分为实验组(11只)和对照组(4只),实验组采用外耳道结扎法构建EACC模型。术后3个月取成模实验小鼠和对照组小鼠外耳道组织,行RT-qPCR、Western blot、ELISA检测IL-4、TREM-2、IL-6、IL-8、IL-1β、肿瘤坏死因子-α(TNF-α)的mRNA和蛋白的表达水平。结果实验组术后3个月造模成功率为91%(10/11);与对照组相比,实验组小鼠EACC组织中TREM-2、IL-6、IL-8、IL-1β、TNF-α的mRNA和蛋白表达水平均显著升高(P<0.05),而IL-4的表达水平显著降低(P<0.05)。结论IL-4/TREM-2信号通路及其介导的炎症反应可能参与小鼠EACC的发生发展。
Objective To investigate the expression and significance of interleukin-4(IL-4)/triggering receptor expressed on myeloid cells-2(TREM-2)signaling pathway-related factors in the tissues of mouse external auditory canal cholesteatoma(EACC).Methods Fifteen C57BL6/J mice were randomly assigned to an experimental group(n=11)and a control group(n=4).EACC model was established in the experimental group via external auditory canal ligation.Three months after the surgery,the external auditory canal tissues were collected from the mice of the experimental and the control group for real-time quantitative polymerase chain reaction,Western blot and enzyme-linked immunosorbent assay to detect the mRNA and protein expression levels of IL-4,TREM-2,IL-6,IL-8,IL-1βand tumor necrosis factor-α(TNF-α).Results The modeling success rate in the experimental group 3 months after surgery was 91%(10/11).Compared with the control group,the mRNA and protein expression levels of TREM-2,IL-6,IL-8,IL-1β,and TNF-αin the EACC tissues of the experimental group were significantly increased(P<0.05),while the expression level of IL-4 was markedly reduced(P<0.05).Conclusion The IL-4/TREM-2 signaling pathway and the inflammatory response it mediates may be involved in the occurrence and development of EACC in mice.
作者
郭秀琴
郑杨帆
卢雯娴
邹亚纯
郑佳怡
何观文
GUO Xiuqin;ZHENG Yangfan;LU Wenxian;ZOU Yachun;ZHENG Jiayi;HE Guanwen(Ningde Clinical Medical College,Fujian Medical University,Ningde 352101,China;Department of Otolaryngology,Ningde Municipal Hospital Affiliated to Ningde Normal University,Ningde 352100,China;Department of Otolaryngology,Pingnan County Hospital,Ningde 352300,China)
出处
《中国耳鼻咽喉颅底外科杂志》
2025年第6期24-28,共5页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
福建省自然基金面上项目(2023J011079)
宁德师范学院医校融合发展专项重点项目(2024ZX71)。
