摘要
[目的]为提高实验室检验能力水平,优化实验数据处理流程,强化实验室质量控制体系,提高实验结果的可信度,本研究对乳酸菌数检验能力进行了系统性验证。[方法]依据《ACAS-PT1911(2024)乳粉中乳酸菌检验能力验证参试指导书》及《GB 4789.35—2023食品安全国家标准食品微生物学检验乳酸菌检验》,对编号24-S614和24-F143的能力验证样品进行检测,并评估不确定度。[结果]编号24-S614样品检测结果为7.0×10^(6),Z值为-1.0;编号24-F143样品检验结果为1.1×10^(7),Z值为1.2。2个检验能力验证样品的│Z│均≤2.0,为满意结果。关键控制点分析及不确定度评估表明稀释误差、称量误差、温度波动、重复检测为主要贡献源,乳酸菌计数结果扩展不确定度为2.2×10^(-4)(P=95%,k=2)。[结论]能力验证的结果表明,实验室在乳酸菌检验能力方面得到了有效提升,增强了实验室检测结果的可信度,证实了此类基质能力验证样品具有良好的稳定性及可靠性,为乳粉中乳酸菌检测方法的标准化提供了参考。
[Objective]To enhance the laboratory testing capability and level,optimize the experimental data processing procedure,strengthen the laboratory quality control system,and improve the credibility of experimental results,this study systematically verified the detection capability of lactic acid bacteria count.[Method]According to the“ACAS-PT1911(2024)Proficiency Testing Guide for Lactic Acid Bacteria in Milk Powder”and“GB 4789.35-2023 National Food Safety Standard-Microbiological testing of food-Lactic Acid Bacteria Testing”,the proficiency testing samples with numbers 24-S614 and 24-F143 were tested,and the uncertainty was evaluated.[Result]The detection result of sample 24-S614 was 7.0×10^(6),with a Z value of-1.0.The detection result of sample 24-F143 was 1.1×10^(7),with a Z value of 1.2.The│Z│of both test proficiency testing samples was≤2.0,which was a satisfactory result..Critical control point analysis and uncertainty assessment indicated that dilution error,weighing error,temperature fluctuation,and repeated detection were the main contributing sources.The expanded uncertainty of the lactic acid bacteria count results was 2.2×10^(-4)(P=95%,k=2).[Conclusion]The results of the proficiency testing indicate that the laboratory's ability to detect lactic acid bacteria has been effectively enhanced,increasing the reliability of the laboratory's detection results.This confirmed that such matrix proficiency testing samples had good stability and reliability,providing a reference for the standardization of lactic acid bacteria detection methods in milk powder.
作者
陈晨
张慧萍
张景泽
靳伟
李振侠
李瑞雪
金箫
CHEN Chen;ZHANG Huiping;ZHANG Jingze;JIN Wei;LI Zhenxia;LI Ruixue;JIN Xiao(Inner Mongolia Mengniu Dairy(Group)Co.,Ltd.,Hohhot Inner Mongolia 011517)
出处
《中国乳业》
2025年第12期71-76,84,共7页
China Dairy
关键词
乳酸菌
检验能力验证
质量控制
lactic acid bacteria
detection proficiency verification
quality control
