摘要
为探明氟污染对水土系统细菌群落的影响,本研究于2022年在宁夏清水河流域采集地表水、沉积物及河岸带土壤样本66组,按地表水氟浓度划分为低氟组(F^(-)<1.0 mg/L)、高氟Ⅰ组(1.0≤F^(-)<1.5 mg/L)和高氟Ⅱ组(F^(-)≥1.5 mg/L),通过16S rDNA高通量测序及多态氟形态分析,系统解析了氟污染下细菌群落的响应特征。结果表明:氟污染降低了细菌α多样性(低氟组>高氟Ⅰ组>高氟Ⅱ组),但沉积物Chao1指数因稀有物种生态位释放而升高。弯曲杆菌门(Campilobacterota)和脱硫杆菌门(Desulfobacterota)被鉴定为耐氟菌门,其中脱硫杆菌门(Desulfobacterota)的物种以低丰度参与硫酸盐还原缓解氟毒性。硫卵菌属(Sulfurovum)等是水土系统中的耐氟细菌属。细菌参与下沉积物/土壤中的残余固定态氟(Res-F)主要通过分解转化为其他形态,土壤环境更有利于细菌对Res-F的分解。本研究首次建立了清水河流域细菌群落与多态氟的关联框架,加深了对河流生态系统中氟化物对细菌群落影响的理解,为制定切实有效的生态环保政策提供了科学依据,有助于阻断或减轻氟化物对生态环境的潜在风险。
The study was conducted with the objective of investigating the impact of fluoride pollution on bacterial communities in the water-soil system.Sixty-six sets of surface water,sediment,and riparian soil samples were collected from the Qingshui River Basin in Ningxia in 2022.The samples were categorized into three distinct groups based on the fluoride concentration in the surface water:low fluoride group(F^(-)<1.0 mg/L),high fluorideⅠgroup(1.0≤F^(-)<1.5 mg/L),and high fluorideⅡgroup(F^(-)≥1.5 mg/L).The bacterial community responses were systematically analyzed through 16S rDNA high-throughput sequencing and polymorphic fluoride species analysis.The results indicated that fluoride pollution led to a decrease in bacterialα-diversity(low fluoride group>high fluorideⅠgroup>high fluorideⅡgroup),although the Chao1 index of sediments increased due to niche release by rare species.The phyla Campilobacterota and Desulfobacterota were identified as fluoride-tolerant bacterial phyla,with species of Desulfobacterota participating in sulfate reduction to alleviate fluoride toxicity at low abundances.Genera such as Sulfurovum have been identified as fluoride-tolerant bacterial genera within the water-soil system.The conversion of residual fixed fluoride(Res-F)in sediments and soils was facilitated by bacteria,who accomplished this by decomposing and transforming it into other forms.It was found that soil environments were more favourable for bacterial decomposition of Res-F.This study establishes the first framework linking bacterial communities to polymorphic fluoride in the Qingshui River Basin,thereby enhancing the understanding of the impact of fluoride on bacterial communities in river ecosystems.The scientific basis for the development of effective ecological and environmental protection policies is provided,thus contributing to the mitigation or reduction of the potential risks of fluoride pollution to the ecological environment.
作者
赵增锋
邱小琮
尹娟
赵睿智
杨强强
Zhao Zengfeng;Qiu Xiaocong;Yin Juan;Zhao Ruizhi;Yang Qiangqiang(School of Civil and Hydraulic Engineering,Ningxia University,Yinchuan 750021,P.R.China;School of Life Science,Ningxia University,Yinchuan 750021,P.R.China;Ningxia Environment Monitoring Center,Yinchuan 750000,P.R.China)
出处
《湖泊科学》
北大核心
2026年第1期193-205,共13页
Journal of Lake Sciences
基金
宁夏自然科学基金一般项目(2025AAC030142)
宁夏自然科学基金重点项目(2023AAC02026)
宁夏高等学校一流学科建设(水利工程)项目(NXYLXK2021A03)联合资助。
关键词
清水河
水土系统
氟化物
细菌
Qingshui River
soil-water system
fluoride
bacteria
