摘要
目的探究地西他滨通过调控生物钟蛋白TIMELESS对乳腺癌细胞生物学特征、能量代谢及CD44-Smad1通路的作用。方法将MCF-7细胞分为MCF-7组、DAC1组、DAC2组与DAC3组。集落形成实验检测细胞增殖能力;Transwell实验检测细胞侵袭能力;比较细胞中ATP水平、LD浓度、LDH活力、细胞葡萄糖摄取量、葡萄糖转运1(GLUT1)蛋白、CD44蛋白和Smad1蛋白表达;RT-PCR检测细胞中TIMELESS mRNA表达。将MCF-7细胞分为sh-NC组与sh-TIM组,比较各组细胞生物学特征、能量代谢和CD44、Smad1蛋白表达。结果地西他滨可抑制细胞克隆数量、侵袭数量、葡萄糖摄取量、ATP水平、LD浓度、LDH活力及GLUT1、CD44、Smad1、TIMELESS表达。抑制TIMELESS表达可抑制细胞克隆数、侵袭数量、葡萄糖摄取量、ATP水平、LD浓度、LDH活力及GLUT1、CD44和Smad1蛋白表达。结论地西他滨通过抑制生物钟蛋白TIMELESS的表达抑制乳腺癌细胞增殖、侵袭和能量代谢,调控乳腺癌细胞中CD44-Smad l通路。
Objective To investigate the role of decitabine on the biological characteristics,energy metabolism and CD44-Smadl pathway of breast cancer cells through the regulation of the biological clock protein TIMELESS.Methods MCF-7 cells were divided into MCF-7 group,DAC1 group,DAC2 group,and DAC3 group.Colony formation test and Transwell test were used to detect the proliferation and invasion ability of cells,respectively;the ATP level,LD concentration,LDH activity,intracellular glucose uptake,GLUT1,CD44 and Smad1 protein expression in cells were detected;RT-PCR was used to detect the expression of TIMELESS in the cells.MCF-7 cells were divided into sh-NC group and sh-TIM group.The biological characteristics,energy metabolism,and expression of CD44 and Smadl proteins in each group were compared.Results Decitabine can inhibit the number of cell clones,the number of invasions,glucose uptake,ATP level,LD concentration,LDH activity and GLUT1,CD44 and Smad1,TIMELESS protein expression.Inhibition of TIMELESS expression can inhibit cell clone count,invasion number,glucose uptake,ATP level,LD concentration,LDH activity and GLUT1,CD44,Smad1 proteins expression.Conclusion Decitabine inhibits breast cancer cell proliferation,invasion and energy metabolism by inhibiting the expression of the biological clock protein TIMELESS,and regulates the CD44 Smadl pathway in breast cancer cells.
作者
周宇
孔祥光
魏于丰
曹丰竹
ZHOU Yu;KONG Xiang-guang;WEI Yu-feng;CAO Feng-zhu(Department of Oncology,Beijing Hospital for the Geriatric,Beijing 100095,China)
出处
《解剖科学进展》
2025年第5期721-725,共5页
Progress of Anatomical Sciences
基金
国家自然科学基金(81603583)。
