摘要
目的探究低强度超声(LIUS)联合微泡(MBs)对结直肠癌恶性进展和免疫逃逸的影响及机制。方法将SW480细胞随机分为Control组、LIUS+MBs 60 s组、LIUS+MBs 120 s组和LIUS+MBs 240 s组。CCK-8检测各组细胞增殖活性以及各组细胞共培养的CD8^(+)T细胞的细胞活力;流式细胞术检测各组细胞以及共培养的CD8^(+)T细胞的凋亡率;Transwell实验检测各组细胞迁移和侵袭能力;Western blot检测各组细胞中PD-L1、p-JNK和p-c-Jun蛋白表达。结果低强度超声联合微泡降低结直肠癌细胞的增殖活性、迁移和侵袭细胞数,增加细胞凋亡率,降低细胞中PD-L1、p-JNK和p-c-Jun蛋白表达,增加与结直肠癌细胞共培养的CD8^(+)T细胞的细胞活力,并降低细胞的凋亡率。结论低强度超声联合微泡抑制结直肠癌细胞增殖、迁移、侵袭和免疫逃逸并诱导细胞凋亡,其机制与抑制JNK/c-Jun信号通路激活介导的PD-L1表达下调有关。
Objective To explore the effects and mechanism of low intensity ultrasound(LIUS)combined with microbubbles(MBs)on malignant progression and immune escape of colorectal cancer.Methods SW480 cells were randomly divided into control group,LIUS+MBs 60 s group,LIUS+MBs 120 s group and LIUS+MBs 240 s group.CCK-8 was used to detect the proliferation activity of cells in each group and the cell viability of CD8^(+)T cells co-cultured in each group.Flow cytometry was used to detect the apoptosis rates of cells in each group and co cultured CD8^(+)T cells.The Transwell assay was used to detect the migration and invasion abilities of cells in each group.Western blot was used to detect the expressions of PD-L1,p-JNK and p-c-Jun in cells of each group.Results Low-intensity ultrasound combined with microbubbles could reduce the proliferation activity,migration and invasion of colorectal cancer cells,increase the apoptosis rate,reduce the expressions of PD-L1,p JNK and p c Jun in cells,and also increase the viability of CD8^(+)T cells co cultured with colorectal cancer cells and reduce the apoptosis rate.Conclusion Low intensity ultrasound combined with microbubbles inhibits the proliferation,migration,invasion,immune escape and induces apoptosis of colorectal cancer cells,and its mechanism is related to the inhibition of the down regulation of PD-L1 expression mediated by the activation of the JNK/c Jun signaling pathway.
作者
聂家鑫
吴昊
张代辉
NIE Jia-xin;WU Hao;ZHANG Dai-hui(Functional Department,Xingtai Central Hospital,Xingtai 054001,China;Medical Imaging Center,Xingtai Central Hospital,Xingtai 054001,China)
出处
《解剖科学进展》
2025年第5期627-630,共4页
Progress of Anatomical Sciences
基金
邢台市重点研发计划项目(2023ZC093)。
