摘要
探究以多齿席夫碱配体H_(2)L与三(二苯甲酰甲烷)合铒(Ⅲ)二水合物[Er(dbm)_(3)·2H_(2)O]制备的双核稀土配合物对尖孢镰刀菌的抑菌效果,为防治土传病害提供理论依据.采用溶剂热合成法制备新型双核稀土配合物,通过菌丝体生长率法和比浊法测定其对尖孢镰刀菌的抑制活性;运用紫外-可见分光光度滴定法、电化学循环伏安法及荧光淬灭法研究配合物与小牛胸腺DNA的相互作用机制.结果显示,浓度为160μg/mL、120μg/mL、60μg/mL的配合物在第5d对尖孢镰刀菌的抑制率分别为77.2%、66.2%和46%,抑制率呈显著剂量依赖性递增(P<0.05),且相同浓度下抑菌率均高于配体及稀土盐;机制研究表明,配合物与DNA的结合方式主要为插入结合.该双核稀土配合物对尖孢镰刀菌具有优异的抑菌活性,其作用机制与DNA插入结合相关.本研究为新型抗菌金属配合物药物的设计与合成提供了重要参考.
This study investigates the antibacterial effect of a binuclear rare earth complex prepared from the multidentate Schiff base ligand H2L and tris(dibenzoylmethane)erbium(Ⅲ)dihydrate[Er(dbm)_(3)·2H_(2)O]on Fusarium oxysporum,providing a theoretical basis for the prevention and control of soil borne diseases.A novel dual core rare earth complex was prepared by solvothermal synthesis method,and its inhibitory activity against Fusarium oxysporum was determined by mycelial growth rate method and turbidity method;The interaction mechanism between the complex and calf thymus DNA was studied using UV visible spectrophotometric titration,electrochemical cyclic voltammetry,and fluorescence quenching methods.The results showed that the complexes with concentrations of 160μg/mL,120μg/mL,and 60μg/mL had inhibition rates of 77.2%,66.2%,and 46%against Fusarium oxysporum on the fifth day,respectively.The inhibition rates showed a significant dose-dependent increase(P<0.05),and were higher than those of ligands and rare earth salts at the same concentration;Mechanism studies have shown that the binding mode between the complex and DNA is mainly insertion binding.The dual core rare earth complex exhibits excellent antibacterial activity against Fusarium oxysporum,and its mechanism of action is related to DNA insertion binding.This study provides important references for the design and synthesis of novel antibacterial metal complex drugs.
作者
贺佳
燕平梅
HE Jia;YAN Pingmei(College of Biological Science and Technology,Taiyuan Normal University,Jinzhong Shanxi 030619,China)
出处
《太原师范学院学报(自然科学版)》
2025年第4期57-62,共6页
Journal of Taiyuan Normal University(Natural Science Edition)
基金
土壤消毒、活化绿色产业技术创新战略联盟.
关键词
稀土配合物
尖孢镰刀菌
菌丝体生长率法
DNA作用
rare earth complexes
fusarium oxysporum
mycelial growth rate method
DNA interaction
