摘要
为获取具有较强富硒能力的酵母菌株,并从富硒酵母中分离生物有机硒以制备富硒食品,本研究采用微生物分离技术,结合耐硒筛选与红硒法进行菌株选育,从富硒地区土壤及果皮样本中分离出1株具有优良硒代谢特性的酵母菌株,经鉴定为库德里阿兹威毕赤酵母(Pichia kudriavzevii)。通过紫外诱变及发酵条件优化,使该菌株菌液富硒量提升至1678 mg/L,硒转化率达98.6%。最后,利用高压冷冻细胞破碎技术裂解酵母细胞,提取生物有机硒,并将其添加至酸奶与饼干中制备富硒食品。本研究可为富硒微生物资源开发及功能性产品应用提供技术依据与数据基础。
This study aimed to obtain yeast strains with strong selenium-enriching capacity and to isolate bioorganic selenium from them for preparing selenium-enriched foods.Microbial isolation techniques,selenium tolerance screening and the tellurite reduction assay were employed.A yeast strain with excellent selenium metabolism characteristics was selected from 12 strains isolated from selenium-enriched soil or fruit peel samples and was identified as Pichia kudriavzevii.After ultraviolet mutagenesis and fermentation condition optimization,the selenium accumulation in this strain increased to 1678 mg/L,with a selenium conversion rate of 98.6%.Finally,the yeast cells were lysed by high-pressure freeze disruption before the extraction of bioorganic selenium,which was incorporated into yogurt and biscuits to produce selenium-fortified foods.This study provides technical references and a data foundation for developing selenium-enriched microbial resources and their application in functional products.
作者
刘杨
马成章
胡玮航
陶鑫
徐飞
LIU Yang;MA Chengzhang;HU Weihang;TAO Xin;XU Fei(Center of Applied Biotechnology,Wuhan University of Bioengineering,Wuhan 430415,China;College of Food Science and Technology,Wuhan University Bioengineering,Wuhan 430415,China)
出处
《食品科学》
北大核心
2025年第24期181-188,共8页
Food Science
基金
湖北省教育厅科学研究项目(B2024296)。
关键词
硒
富硒酵母
诱变育种
富硒食品
selenium
selenium-enriched yeast
mutagenesis-based breeding
selenium-rich foods
